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Series GSE111216 Query DataSets for GSE111216
Status Public on Apr 04, 2019
Title Mouse transcriptomics reveals extracellular matrix organization as a major pathway involved in inflammatory and neuropathic pain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Chronic pain is a debilitating and poorly-treated condition. The mechanisms underlying the development of chronic pain are not well understood. Nerve injury and inflammation cause alterations in gene expression in tissues associated with transmission of pain, supporting molecular and cellular mechanisms that maintain painful states. Previous studies in animal models and human patients suffering from different chronic pain conditions have examined changes in transcriptome associated with chronic pain. However, in most studies, the analyses were restricted to a single tissue or pain condition. In the current study, we performed next-generation sequencing of dorsal root ganglia, spinal cord, brain and blood in mouse models of nerve injury and inflammation-induced chronic pain. Comparative analyses of differentially expressed genes (DEG) across these tissues in two pain models identified the extracellular matrix organization (ECMO) pathway as the most commonly affected pathway. Interestingly, examination of GWAS datasets revealed an over-representation of DEGs within the ECMO pathway in SNPs most strongly associated with human back pain. Remarkably, manipulation of the extracellular matrix in the mouse significantly affected the development of pain hypersensitivity following nerve injury, supporting our gene expression findings. In summary, our comprehensive analysis of transcriptional landscape across different mouse pain models and tissues as well as human GWAS datasets identified extracellular matrix organization as a central molecular pathway in the development of chronic pain.
Overall design Complete Freund's Adjuvant (CFA) and Spared Nerve Injury (SNI) were performed on mice. CFA is surrogate for inflammation pain, while SNI for neuropathic pain. Untreated mice were used as controls. Mice tissues were collected. Transcriptomes were compared, in a tissue-based and pain-related manner.
Contributor(s) Parisien M, Samoshkin A, Tansley SN, Piltonen MH, Martin L, Mogil JS, Khoutorsky A, Diatchenko L
Citation(s) 30763288
Submission date Feb 27, 2018
Last update date Apr 04, 2019
Contact name Marc Parisien
Organization name McGill University
Department Dentistry
Lab Human Pain Genetics Lab
Street address 740 Dr. Penfield Avenue
City Montreal
State/province Quebec
ZIP/Postal code H3A 0G1
Country Canada
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (36)
GSM3025457 BRN_CTR_rep1
GSM3025458 BRN_CTR_rep2
GSM3025459 BRN_CTR_rep3
BioProject PRJNA436177
SRA SRP133622

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Supplementary file Size Download File type/resource
GSE111216_Parisien_Suppl_Table_01_gene_BRN_CTRvsCFA.xlsx 3.6 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_02_gene_BRN_CTRvsSNI.xlsx 3.6 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_03_gene_SC_CTRvsCFA.xlsx 3.5 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_04_gene_SC_CTRvsSNI.xlsx 3.5 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_05_gene_DRG_CTRvsCFA.xlsx 3.5 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_06_gene_DRG_CTRvsSNI.xlsx 3.5 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_07_gene_BLD_CTRvsCFA.xlsx 2.9 Mb (ftp)(http) XLSX
GSE111216_Parisien_Suppl_Table_08_gene_BLD_CTRvsSNI.xlsx 3.0 Mb (ftp)(http) XLSX
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