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Series GSE111107 Query DataSets for GSE111107
Status Public on Apr 27, 2018
Title A single-cell transcriptome atlas of mouse glomerulus
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Three different cell types constitute the glomerular filter: mesangial cells, endothelial cells, and podocytes. As yet, it remains unknown to what extent cellular heterogeneity exists within healthy glomerular cell populations. Here, we used nanodroplet-based, highly parallel transcriptional profiling to characterize the cellular content of purified wildtype mouse glomeruli. Unsupervised clustering of 13,000 single-cell transcriptomes identified the three known glomerular cell types. We provide a comprehensive online atlas of gene expression in glomerular cells, which can be queried and visualized using an interactive and freely available database. Novel marker genes for all glomerular cell types were identified and supported by immunohistochemistry stainings obtained from the Human Protein Atlas. Subclustering of glomerular endothelial cells revealed a subset of activated endothelium, expressing marker genes related to endothelial proliferation. Additionally, the podocyte population could be divided in three different subclusters. In conclusion, our study comprehensively characterizes gene expression in individual glomerular cells and sets the stage for the dissection of glomerular function at the single-cell level in health and disease.
Overall design Single cell transcriptomics of Mouse Kidney Glomeruli using Drop-seq
Contributor(s) Karaiskos N, Rahmatollahi M, Kocks C, Kann M, Mueller R
Citation(s) 29794128
Submission date Feb 26, 2018
Last update date Mar 25, 2019
Contact name Nikos Karaiskos
Organization name Max Delbrück Center for Molecular Medicine
Lab Systems Biology of Gene Regulatory Elements
Street address Hannoversche Str. 28
City Berlin
ZIP/Postal code 10115
Country Germany
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM3022239 glom_rep1
GSM3022240 glom_rep2
GSM3022241 glom_rep3
BioProject PRJNA435940
SRA SRP133475

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111107_RAW.tar 15.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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