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Series GSE111035 Query DataSets for GSE111035
Status Public on Jun 20, 2019
Title Maternal Plag1 deficiency delays two-cell stage embryo development and embryonic genome activation [Uterus]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Pleomorphic adenoma gene 1 (PLAG1) encodes a transcription factor involved in cancer and growth. We study the role of PLAG1 in female reproductive tract and preimplantation embryos using STRT RNA-seq of single embryos from wild type and knockout mothers (both mated with wild type studs). We did not find significant effects in ovaries or uterus. The lack of maternal Plag1 led to delayed mouse 2-cell stage embryo development, compensatory expression of Plag1 from the paternal allele, and dysregulation of 1,089 genes. Half of these genes displayed a pattern of delayed activation and play roles in ribosome biogenesis and protein synthesis. These mouse genes further showed a significant overlap with human EGA genes with similar ontology, and an enrichment of the PLAG1 de novo motif. We conclude that Plag1 affects EGA through retrotransposons influencing ribosomes and protein synthesis, a mechanism that might also explain its roles in cancer and growth
Overall design Uterus was dissected from 8 WT and 8 Plag1KO mice that were used in superovulation experiments and used for RNA-seq. One uterine horn was split longitudinally and mucosa exposed. The endometrial cells were scraped with a scalpel. Endometrial cells (ENDO), remaining myometrium (MYO), and a piece of intact uterine horn were transferred to RNA later.
Contributor(s) Damdimopoulou P, Damdimopoulos A, Katayama S, Kere J
Citation(s) 31182756
Submission date Feb 23, 2018
Last update date Jun 20, 2019
Contact name Pauliina Damdimopoulou
Organization name Karolinska Institute
Street address Karolinska University Hospital OBGY K57
City Stockholm
ZIP/Postal code 14186
Country Sweden
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (45)
GSM3020647 ut_WT_1
GSM3020648 ut_KO_2
GSM3020649 ut_WT_3
This SubSeries is part of SuperSeries:
GSE111040 Maternal Plag1 deficiency delays two-cell stage embryo development and embryonic genome activation
BioProject PRJNA435667
SRA SRP133348

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Supplementary file Size Download File type/resource
GSE111035_rawCountsUterus.txt.gz 648.8 Kb (ftp)(http) TXT
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