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| Status |
Public on Jun 06, 2018 |
| Title |
The lack of α1,3-fucosyltransferase (Osfuct) affects in anther and pollen development in rice (Oryza sativa) |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by genome tiling array
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| Summary |
The profiling was conducted with the Rice 3'-Tiling 135k Microarray designed from 31,439 genes deposited at IRGSP, RAP2 database (http://rapdb.lab.nig.ac.jp).
We have identified and characterized a T-DNA insert rice mutant (Osfuct) with loss of α1,3-fucosyltransferase function. Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycan revealed the lack of α1,3-fucose in the N-glycan structure of rice Osfuct mutant. The mutant displayed the pleiotropic developmental defects such as diminished growth, shorter plant height, less number of tillers, shorter panicle lengths and internode, impaired anther and pollen development. In addition, the anther was curved, pollen grains shapes were shriveled, pollen viability and pollen number per anther was dramatically decreased in Osfuct mutant. The complementation test of Osfuct mutant clearly exhibited that the phenotype is caused by the loss of α1,3-fucosyltransferase function bescause complementation line is rescued. Transcriptome profiling data revealed that several genes essential in plant developmental processes were significantly altered in Osfuct mutant including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis and hypothetical proteins. Moreover, Osfuct mutant exhibited the enhanced salt insensitivity. Taken together, these findings demonstrated that Osfuct plays a critical role in growth, anther, pollen development and salt stress response.
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| Overall design |
Experimental group : alpha1,3-fucosyltransferase gene knock-out rice (three biological replicates) Control group : Dongjin (three biological replicates)
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| Contributor(s) |
Hahn B, Sim J, Singh Kesawat M, Lee C, Park S |
| Citation(s) |
29670011 |
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| Submission date |
Feb 20, 2018 |
| Last update date |
Jun 06, 2018 |
| Contact name |
Yeon-Ki Kim |
| E-mail(s) |
kim750a11@gmail.com
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| Phone |
82-31-321-6351
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| Organization name |
GreenGene Biotech Inc.
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| Department |
GreenGene Biotech Inc.
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| Lab |
Genomics & Genetics Ins.
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| Street address |
38-2 Namdong
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| City |
Yongin |
| State/province |
Kyonggido |
| ZIP/Postal code |
449-728 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA434720 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE110873_RAW.tar |
30.0 Mb |
(http)(custom) |
TAR (of PAIR, PROBE) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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