Other Genome binding/occupancy profiling by high throughput sequencing
Summary
Human accelerated regions (HARs) are evolutionarily conserved sequences that acquired human-specific nucleotide changes and reside in genomic regions associated with unique human traits and disease. The majority of HARs (96%) are noncoding, a few of which have been shown to be functional enhancers. Here, we comprehensively tested human and chimpanzee sequences of HARs (N=714) for enhancer activity using a lentivirus-based massively parallel reporter assay (lentiMPRA) in human and chimpanzee iPSC derived neural progenitors at two differentiation time points. We found that 43% (306/714) function as enhancers and over two-thirds (204/306) showed consistent differences in activity between human and chimpanzee sequences across conditions. We also tested all possible permutations of substitutions in seven HARs and found significant positive and negative interactions. Our study provides a comprehensive resource of functional neurodevelopmental HAR enhancers and shows that multiple interacting sites drive evolutionary activity differences.
Overall design
We applied a lentivirus-based massively parallel reporter assay (lentiMPRA) to directly compare the enhancer activities of the human and chimpanzee alleles of 714 human-accelerated regions in human and chimpanzee iPSC-derived neural progenitor cell lines (N2 and N3 cell stages).
Pt2A and Pt5C refer to two iPSC cell lines, each derived from a different individual.
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