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Series GSE110655 Query DataSets for GSE110655
Status Public on Aug 02, 2018
Title Binding of TMPRSS2-ERG to BAF Chromatin Remodeling Complexes Mediates Prostate Oncogenesis.[ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Chromosomal rearrangements resulting in the fusion of TMRPSS2, an androgen-regulated gene, and the ETS family transcription factor ERG occur in over half of prostate cancers. However, the mechanism by which ERG promotes oncogenic gene expression and proliferation remains incompletely understood. Here, we identify a binding interaction between ERG and the mammalian SWI/SNF (BAF) ATP-dependent chromatin remodeling complex, which is conserved among other ETS factors, including ETV1, ETV4, and ETV5. We find that ERG drives genome-wide retargeting of BAF complexes in a manner dependent on binding of ERG to the ETS DNA motif. Moreover, ERG requires intact BAF complexes for chromatin occupancy and BAF complex ATPase activity for target gene regulation. In a prostate organoid model, BAF complexes are required for ERG-mediated basal-to-luminal transition, a hallmark of ERG activity in prostate cancer. These observations suggest a fundamental interdependence between ETS transcription factors and BAF chromatin remodeling complexes in cancer.
 
Overall design Prostate cancer cell lines (VCaP, LNCaP) were lentivirally infected with respective expression/knockdown constructs, selected, and harvested for ChIP-seq.
 
Contributor(s) Pulice J, Kadoch C
Citation(s) 30078722
Submission date Feb 15, 2018
Last update date Mar 26, 2019
Contact name Cigall Kadoch
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Kadoch Lab
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (26)
GSM3223705 VCaP shCt ERG
GSM3223706 VCaP shERG ERG
GSM3223707 VCaP shARID1A ERG
This SubSeries is part of SuperSeries:
GSE110657 Binding of TMPRSS2-ERG to BAF Chromatin Remodeling Complexes Mediates Prostate Oncogenesis.
Relations
BioProject PRJNA434201
SRA SRP132914

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Supplementary file Size Download File type/resource
GSE110655_RAW.tar 28.1 Mb (http)(custom) TAR (of BROADPEAK, NARROWPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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