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| Status |
Public on Feb 27, 2009 |
| Title |
Temporal expression analysis of C. elegans larvae hatching in the presence and absence of food. |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
When C. elegans larvae hatch in the absence of food they persist in a stress resistant, developmentally arrested state (L1 arrest). We characterized mRNA expression genome-wide in a pair of bifurcating time series starting in the late embryo and proceeding through the hatch in the presence and absence of food (E. coli).
We used Affymetrix C. elegans expression arrays to measure gene expression in 18 total timepoint/conditions in three biological replicates.
Keywords: time course; plus/minus food
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| Overall design |
Nematode populations were staged such that they hatched withing a 2.5 hr window, and time point zero (0 hr) corresponds to when 50% of the population had hatched. RNA was isolated with TRIzol and 100 ng was used for T7 RNA polymerase-based mRNA amplification and biotin labeling using MessageAmp II-Biotin Enhanced kit (Ambion). Labeled cRNA was fragmented, hybridized to the Affymetriz array, and scanned according to the manufacturer’s protocol.
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| Contributor(s) |
Baugh LR, Sternberg PW |
| Citation(s) |
19251593, 21445366 |
| Submission date |
Apr 04, 2008 |
| Last update date |
Jul 06, 2016 |
| Contact name |
L. Ryan Baugh |
| E-mail(s) |
ryan.baugh@duke.edu
|
| Phone |
919-613-8179
|
| Organization name |
Duke University
|
| Department |
Biology
|
| Lab |
Baugh
|
| Street address |
4314 French Family Science Center
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708-0338 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
|
| Samples (18)
|
| GSM279203 |
embryo 2 hr before hatching |
| GSM279204 |
L1 larvae 0 hr after hatching in the absence of food |
| GSM279205 |
L1 larvae 1 hr after hatching in the absence of food |
| GSM279206 |
L1 larvae 3 hr after hatching in the absence of food |
| GSM279207 |
L1 larvae 6 hr after hatching in the absence of food |
| GSM279208 |
L1 larvae 12 hr after hatching in the absence of food |
| GSM279209 |
L1 larvae 15 hr after hatching in the absence of food |
| GSM279210 |
L1 larvae 24 hr after hatching in the absence of food |
| GSM279211 |
L1 larvae 0 hr after hatching in the presence of food |
| GSM279212 |
L1 larvae 1 hr after hatching in the presence of food |
| GSM279213 |
L1 larvae 3 hr after hatching in the presence of food |
| GSM279214 |
L1 larvae 6 hr after hatching in the presence of food |
| GSM279215 |
L1 larvae 12 hr after hatching in the presence of food |
| GSM279216 |
L1-L2 larvae 15 hr after hatching in the presence of food |
| GSM279217 |
L2 larvae 24 hr after hatching in the presence of food |
| GSM279218 |
L1 larvae 3 hr after hatching in the absence of food in the alternative starvation buffer M9 |
| GSM279219 |
L1 larvae starved for 12 hr after hatching and then fed for 3 hr |
| GSM279220 |
L1 larvae fed for 12 hr after hatching and then starved for 3 hr |
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| This SubSeries is part of SuperSeries: |
| GSE14009 |
Nutritional control of gene expression during C. elegans L1 arrest and recovery |
|
| Relations |
| BioProject |
PRJNA113821 |
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