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Series GSE11055 Query DataSets for GSE11055
Status Public on Feb 27, 2009
Title Temporal expression analysis of C. elegans larvae hatching in the presence and absence of food.
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary When C. elegans larvae hatch in the absence of food they persist in a stress resistant, developmentally arrested state (L1 arrest). We characterized mRNA expression genome-wide in a pair of bifurcating time series starting in the late embryo and proceeding through the hatch in the presence and absence of food (E. coli).
We used Affymetrix C. elegans expression arrays to measure gene expression in 18 total timepoint/conditions in three biological replicates.
Keywords: time course; plus/minus food
 
Overall design Nematode populations were staged such that they hatched withing a 2.5 hr window, and time point zero (0 hr) corresponds to when 50% of the population had hatched. RNA was isolated with TRIzol and 100 ng was used for T7 RNA polymerase-based mRNA amplification and biotin labeling using MessageAmp II-Biotin Enhanced kit (Ambion). Labeled cRNA was fragmented, hybridized to the Affymetriz array, and scanned according to the manufacturer’s protocol.
 
Contributor(s) Baugh LR, Sternberg PW
Citation(s) 19251593, 21445366
Submission date Apr 04, 2008
Last update date Jul 06, 2016
Contact name L. Ryan Baugh
E-mail(s) ryan.baugh@duke.edu
Phone 919-613-8179
Organization name Duke University
Department Biology
Lab Baugh
Street address 4314 French Family Science Center
City Durham
State/province NC
ZIP/Postal code 27708-0338
Country USA
 
Platforms (1)
GPL200 [Celegans] Affymetrix C. elegans Genome Array
Samples (18)
GSM279203 embryo 2 hr before hatching
GSM279204 L1 larvae 0 hr after hatching in the absence of food
GSM279205 L1 larvae 1 hr after hatching in the absence of food
This SubSeries is part of SuperSeries:
GSE14009 Nutritional control of gene expression during C. elegans L1 arrest and recovery
Relations
BioProject PRJNA113821

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11055_FedStarvedExpression.xls.gz 18.3 Mb (ftp)(http) XLS
GSE11055_FedStarvedExpression_ReadMe.txt 819 b (ftp)(http) TXT
GSE11055_RAW.tar 117.0 Mb (http)(custom) TAR (of CEL, CHP, EXP)
GSE11055_WormBaseWS180mapping.txt 428.4 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file
Raw data provided as supplementary file
Processed data are available on Series record

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