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| Status |
Public on Nov 22, 2019 |
| Title |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. [Epigenetics] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Human fetal naïve T cells preferentially differentiate into CD25hiFOXP3hi regulatory T (Treg) cells upon T cell receptor (TCR) stimulation, which contribute to the generation of immunotolerance in the developing human fetus. We utilized ATACseq and H3K27ac ChIPseq to characterize the human fetal naive T cell epigenome relative to that of adult naive T cells and Treg cells. Fetal naive T cells share a partial Treg epigenome with increased chromatin accessibility and H3K27ac enrichment at a subset of Treg-specific enhancers.
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| |
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| Overall design |
ATACseq and H3K27ac ChIPseq for ex vivo sorted adult CD4+CD25-CD45RA+CD27+ naïve T, adult CD4+CD25hiCD127lo regulatory T and fetal naïve T cells from fetal spleen and adult peripheral blood mononuclear cells
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| |
|
| Contributor(s) |
Ng MS, Burt TD |
| Citation(s) |
31757834 |
| |
| Submission date |
Feb 12, 2018 |
| Last update date |
Feb 21, 2020 |
| Contact name |
Melissa Ng |
| E-mail(s) |
melissa.ng@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Lab |
Burt Lab
|
| Street address |
35 Medical Centre Way, Regeneration Medicine Building 903B
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE110472 |
Helios enhances the preferential differentiation of human fetal CD4+ naïve T cells into regulatory T cells. |
|
| Relations |
| BioProject |
PRJNA433824 |
| SRA |
SRP132691 |
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