|
| Status |
Public on Oct 18, 2019 |
| Title |
ChIP-seq analysis to understand the mechanistic role of NCoR1 as a tolerogenic program suppressor in dendritic cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We found in our study that NCoR1 knock down dendritic cells (DCs) develop tolerogenic behavior upon activation and these cells have the potential to modulate T helper cell differentiation toward Treg phenotype. Here to understand the mechanism of this process we performed NCoR1 ChIP-seq in wild type DCs before and after CpG ligand activation and transcriptome analysis of NCoR1 KD DCs to identify the direct and indirect target genes. We also predicted PU.1 as the recruting factor for NCoR1 in DCs based on motif enrichment and later on validated that by performing PU.1 ChIP-seq in control and NCoR1 KD DCs.
|
| |
|
| Overall design |
Mechanistic role of NCoR1 co-repressor in dendritic cell function
|
| |
|
| Contributor(s) |
Ahad A, Smita S, Stevanin M, Mishra GP, Gupta D, Waszak SM, Gupta B, Acha-Orbea H, Raghav SK |
| Citation(s) |
31522122 |
| |
| Submission date |
Feb 09, 2018 |
| Last update date |
Jan 17, 2020 |
| Contact name |
Sunil Kumar Raghav |
| E-mail(s) |
sunilraghav@ils.res.in, j12mishra@gmail.com
|
| Organization name |
Institute of Life Sciences
|
| Department |
Infectious Disease Biology
|
| Lab |
Immunogenomics and Systems Biology Lab
|
| Street address |
Nalco Square
|
| City |
Bhubaneswar |
| State/province |
Odisha |
| ZIP/Postal code |
751023 |
| Country |
India |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (9)
|
|
| This SubSeries is part of SuperSeries: |
| GSE110423 |
RNA-seq and ChIP-seq analysis to understand the mechanistic role of NCoR1 as a tolerogenic program suppressor in dendritic cells |
|
| Relations |
| BioProject |
PRJNA433709 |
| SRA |
SRP132634 |
Less...
More...