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| Status |
Public on Aug 14, 2019 |
| Title |
Impact of G-content on Arabidopsis AtPAB binding and their role in enhancing translational efficiency |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
Expression profiling by high throughput sequencing
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| Summary |
Polyadenylation plays a key role in producing mature mRNAs in eukaryotes. It is widely believed that the poly(A)-binding proteins (PABs) uniformly bind to poly(A)-tailed mRNAs, regulating their stability and translational efficiency. Here, we characterized the RNA-binding landscape of three broadly expressed PABs in Arabidopsis thaliana. We observed substantial variation in the AtPAB-binding efficiency among mRNAs, which can be partly explained by the guanosine (G) content of poly(A) tails. AtPAB-binding efficiency of a gene was positively associated with translational efficiency rather than mRNA stability. Consistently, genes with stronger AtPAB binding exhibited a greater reduction in translational efficiency when AtPAB is depleted. Our study provides a new mechanism that translational efficiency of a gene can be regulated through G-content-dependent PAB binding, paving the way for a better understanding of poly(A) tail-associated regulation of gene expression.
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| Overall design |
CLIP-seq and ribosome profiling were used to study direct binding genes and the functions on translational regulation of AtPABs. Poly(A)-seq was used to detect the mRNA tail information in Col. Degradtion data was used to detect mRNA degradation rate in Col.
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| Contributor(s) |
Zhao T, Sun J, Liu C, Huan Q, Qian W, Cao X |
| Citation(s) |
31481099 |
| |
| Submission date |
Feb 08, 2018 |
| Last update date |
Sep 18, 2019 |
| Contact name |
xiaofeng cao |
| E-mail(s) |
xfcao@genetics.ac.cn
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| Phone |
86-10-64869203
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| Organization name |
Institute of Genetics and Developmental Biology
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| Department |
State Key Laboratory of Plant Genomics
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| Street address |
West Lincui Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (3) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
| GPL23157 |
HiSeq X Ten (Arabidopsis thaliana) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA433501 |
| SRA |
SRP132472 |
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