|
| Status |
Public on Dec 05, 2019 |
| Title |
RNA-seq profiling of FACS purified MPs from adult skeletal muscle after conditional knockout of Hic1 [MP RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine the effect of the deletion of the trancriptional repressor Hypermethylated in cancer 1 (Hic1) on quiescence, adult mesenchymal progenitors (MPs) were isolated from skeletal muscle of both wild type mice and those conditionally deleted for Hic1 and profiled by RNA sequencing. Comparison to MPs activated by using a muscle injury model of the tibialis anterior muscle revealed similar pathways affected under both conditions, highlighting the role for Hic1 in maintaining the quiescent state of MPs
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| |
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| Overall design |
Transcriptome wide profiling of adult mesechymal progenitors from skeletal muscle of wild type and Hic1-/- mice in quadruplicate.
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| |
|
| Contributor(s) |
Scott RW, Underhill TM |
| Citation(s) |
31809738 |
| |
| Submission date |
Feb 01, 2018 |
| Last update date |
Feb 19, 2020 |
| Contact name |
Tully Michael Underhill |
| E-mail(s) |
tunderhi@brc.ubc.ca
|
| Phone |
604-822-5833
|
| Organization name |
University of British Columbia
|
| Department |
Biomedical Research Centre
|
| Street address |
2222 Health Sciences Mall
|
| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V6T 1Z3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
| GSM3932055 |
RNA-seq wildtype replicate 1 [MP RNA-seq] |
| GSM3932056 |
RNA-seq wildtype replicate 2 [MP RNA-seq] |
| GSM3932057 |
RNA-seq wildtype replicate 3 [MP RNA-seq] |
| GSM3932058 |
RNA-seq knockout replicate 1 [MP RNA-seq] |
| GSM3932059 |
RNA-seq knockout replicate 2 [MP RNA-seq] |
| GSM3932060 |
RNA-seq knockout replicate 3 [MP RNA-seq] |
|
| This SubSeries is part of SuperSeries: |
| GSE110038 |
Hic1 defines quiescent mesenchymal progenitor subpopulations with distinct functions and fates in skeletal muscle regeneration |
|
| Relations |
| BioProject |
PRJNA432558 |
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