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Series GSE109891 Query DataSets for GSE109891
Status Public on Mar 13, 2018
Title Clinical and genomic crosstalk between glucocorticoid receptor and estrogen receptor α in endometrial cancer [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Steroid hormone receptors are simultaneously active in many tissues and capable of altering each other's function. Estrogen receptor ɑ (ER) and glucocorticoid receptor (GR) are expressed in the uterus and their ligands have opposing effects on uterine growth. In endometrial tumors expressing high levels of ER, we surprisingly found that expression of GR is associated with poor prognosis. Dexamethasone reduced normal uterine growth in vivo; however, this growth inhibition was abolished in estrogen-induced endometrial hyperplasia. We observed low genomic binding site overlap when ER and GR are induced with their respective ligands; however, upon simultaneous induction they co-occupy more sites. GR binding is significantly altered by estradiol with GR recruited to ER bound loci that become more accessible upon estradiol induction. Gene expression responses to co-treatment were more similar to estradiol, but with novel regulated genes. Our results suggest phenotypic and molecular interplay between ER and GR in endometrial cancer.
 
Overall design ChIP-seq, ATAC-seq, and RNA-seq data collected from endometrial cancer cell lines induced with dexamethasone, estradiol, or the combination
 
Contributor(s) Gertz J, Vahrenkamp J
Citation(s) 29539426
Submission date Jan 30, 2018
Last update date Mar 27, 2019
Contact name Jason Gertz
E-mail(s) jay.gertz@hci.utah.edu
Organization name University of Utah
Department Oncological Sciences
Lab Gertz
Street address 2000 Circle of Hope
City Salt Lake CIty
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (11)
GSM2973394 Ishi_Dex_E2_ChIP_ER
GSM2973395 Ishi_Dex_E2_ChIP_GR
GSM2973396 Ishi_E2_ChIP_ER
This SubSeries is part of SuperSeries:
GSE109893 Clinical and genomic crosstalk between glucocorticoid receptor and estrogen receptor α in endometrial cancer
Relations
BioProject PRJNA432244
SRA SRP131799

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109891_RAW.tar 689.8 Mb (http)(custom) TAR (of BW, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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