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Series GSE109619 Query DataSets for GSE109619
Status Public on Jan 26, 2018
Title Regulation of the positive transcriptional effect of PLZF through a non-canonical EZH2 activity
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The transcription factor PLZF (promyelocytic leukemia zinc finger protein) acts as an epigenetic regulator balancing self-renewal and differentiation of hematopoietic cells through binding to various chromatin-modifying factors. First described as a transcriptional repressor, PLZF is also associated with active transcription, although the molecular bases underlying the differences are unknown. Here, we reveal that, in a hematopoietic cell line, PLZF is predominantly associated with transcribed genes. Additionally, we identify a new association between PLZF and the histone methyltransferase, EZH2 at the genomic level. We find that co-occupancy of PLZF and EZH2 on chromatin at PLZF target genes is not associated with SUZ12 or trimethylated lysine 27 of histone H3 (H3K27me3) but with the active histone mark H3K4me3 and active transcription. Removal of EZH2 leads to an increase of PLZF binding and increased in gene expression. Our results suggest a new role of EZH2 in restricting PLZF positive transcriptional activity independently of its canonical PRC2 activity.
Overall design PLZF, EZH2, SUZ12 and histone marks (H3K27me3, H3K4me3, H3K36me3) were analyzed using ChIPseq in human myeloblastic cell line (KG1) in wild-type (WT), shPLZF or shEZH2 conditions. Each condition represents a merge of two biological replicates.
Contributor(s) Duprez E, Koubi M, Poplineau M, Vernerey J, Nguyen L, Tiberi G, El-Kaoutari A, Garciaz S, Andrau J, Maqbool M, Guillouf C, Saurin A
Citation(s) 29425303
Submission date Jan 25, 2018
Last update date May 22, 2019
Contact name Estelle Duprez
Organization name INSERM
Department Centre de Recherche en Cancérologie de Marseille
Street address 27 boulevard Lei Roure
City Marseille
ZIP/Postal code 13009
Country France
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (15)
GSM2947301 H3K27me3_ChIPSeq_shCtrl
GSM2947302 H3K27me3_ChIPSeq_shPLZF
GSM2947303 H3K27me3_ChIPSeq_shEZH2
BioProject PRJNA431542
SRA SRP131214

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Supplementary file Size Download File type/resource
GSE109619_RAW.tar 3.1 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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