|
| Status |
Public on Jan 24, 2018 |
| Title |
Next Generation Sequencing based on the DNA fragment pulled down by p53 antibody (Santa Cruz, sc-126) from MCF7 wile type and MCF7 MDMX-C463A/WT cells. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Hypothesis: Based on gene chip results and further investigation, MDMX C463A/WT cells shows large portion of transcripts change at p53 dependent manner. As transcriptional factor, p53 regulates those genes could be related to DNA binding status change. ChIP-seq was used to test this hypothesis and figure out global map of p53 binding in MCF7 C463A/WT MCF7 cells by using MCF7 parental cells as reference.
|
| |
|
| Overall design |
Examination of p53 genomic DNA binding affinity change in MCF7 C463A/WT cells comparing to parental cells.
|
| |
|
| Contributor(s) |
Yuan Z, Zeng W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 23, 2018 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Zhi-Min Yuan |
| E-mail(s) |
zyuan@hsph.harvard.edu
|
| Phone |
6174322139
|
| Organization name |
Harvard University
|
| Department |
School of Public Health
|
| Lab |
John B. Little Center
|
| Street address |
Goldenson Building, Long wood Ave.
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02120 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (4)
|
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| This SubSeries is part of SuperSeries: |
| GSE109482 |
Expression data and ChIP-seq from MCF7 wildtype and MCF7 MDMXC463A/WT cells |
|
| Relations |
| BioProject |
PRJNA431182 |
| SRA |
SRP131121 |
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