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Series GSE109241 Query DataSets for GSE109241
Status Public on Feb 14, 2018
Title Rif1 acts through Protein Phosphatase 1 and independent of replication timing to suppresses telomere extension in budding yeast
Organism Saccharomyces cerevisiae
Experiment type Other
Summary The Rif1 protein negatively regulates telomeric TG repeat length in the budding yeast S. cerevisiae, but how it prevents telomere over-extension is unknown. Rif1 was recently shown to control DNA replication by acting as a Protein Phosphatase 1 (PP1)-targeting subunit. Therefore we investigated whether Rif1 controls telomere length by targeting PP1 activity. We find that a Rif1 mutant that cannot interact with PP1 causes a long-telomere phenotype, similar to that of rif1∆ cells. Compromised PP1 function also causes telomere extension. Tethering PP1 at a specific telomere partially substitutes for Rif1 in limiting TG repeat length, confirming the importance of PP1 in telomere length control. Ablating Rif1-PP1 interaction leads to precocious activation of telomere-proximal replication origins and aberrantly early telomere replication. However, we find that Rif1 still limits telomere length even if nearby replication origins are deleted, indicating that effects of Rif1 on telomere length are not mediated through replication timing. Instead we find that, even at a telomere created after DNA synthesis during a mitotic block, Rif1-PP1 interaction is required to suppress telomere lengthening and prevent inappropriate recruitment of Tel1 kinase. Overall, our results show that Rif1 controls telomere length by recruiting PP1 to directly suppress telomerase-mediated TG repeat lengthening.
 
Overall design Saccharomyces cerevisiae strains were arrested in G1 with alpha-factor before being released into S-phase. Copy number was measured by NGS at various timepoints into S-phase, as a proxy for replication timing.
 
Contributor(s) Kedziora S, Hiraga S, Clark KM, Wilson RC, Nieduszynski CA, Donaldson AD
Citation(s) 29529242
Submission date Jan 16, 2018
Last update date Mar 13, 2019
Contact name Conrad A Nieduszynski
Organization name Earlham Institute
Lab DNA replication & genome stability
Street address Norwich Research Park
City Norwich
ZIP/Postal code NR4 7UZ
Country United Kingdom
 
Platforms (1)
GPL19756 Illumina NextSeq 500 (Saccharomyces cerevisiae)
Samples (6)
GSM2935792 GA1459_0
GSM2935793 GA1459_75
GSM2935794 HE61_75
Relations
BioProject PRJNA430235
SRA SRP131009

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Supplementary file Size Download File type/resource
GSE109241_RAW.tar 180.0 Kb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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