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Series GSE10919 Query DataSets for GSE10919
Status Public on Mar 22, 2008
Title Transgenerational epigenetic programming of the embryonic testis transcriptome
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination appears to promote an epigenetic reprogramming of the male germ line that is associated with transgenerational adult-onset disease states. Transgenerational effects on the embryonic day 16 (E16) testis demonstrated reproducible changes in the testis transcriptome for multiple generations (F1-F3). The expression of 196 genes was found to be influenced, with the majority of gene expression being decreased or silenced. Dramatic changes in the gene expression of methyltransferases during gonadal sex determination were observed in the F1 and F2 vinclozolin generation (E16) embryonic testis, but the majority returned to control-generation levels by the F3 generation. The most dramatic effects were on the germ-line-associated Dnmt3A and Dnmt3L isoforms. Observations demonstrate that an embryonic exposure to vinclozolin appears to promote an epigenetic reprogramming of the male germ line that correlates with transgenerational alterations in the testis transcriptome in subsequent generations.
Keywords: expression analysis, transgenerational changes due to Vinclozolin
Overall design E16 Testis RNA samples from F1, F2, F3 generation control groups are compared to F1, F2, F3 generation vinclozolin treated groups
Web link
Contributor(s) Anway M, Rekow S, Skinner M
Citation(s) 18042343
Submission date Mar 21, 2008
Last update date Jul 31, 2017
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (12)
GSM276821 F1-Control embryonic testis day E16, replica 1
GSM276822 F1-Control embryonic testis day E16, replica 2
GSM276823 F2-Control embryonic testis day E16, replica 1
BioProject PRJNA107221

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Supplementary file Size Download File type/resource
GSE10919_RAW.tar 31.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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