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| Status |
Public on Mar 21, 2008 |
| Title |
Bmp2 Is Critical for the Murine Decidual Response |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The role of bone morphogenetic protein 2 (Bmp2)in regulating the transformation of the uterine stroma during embryo implantation in the mouse was investigated by the conditional ablation of Bmp2 in the uterus using the (PR-cre) mouse.
Keywords: Genetic modification
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| Overall design |
Bmp2 gene ablation was confirmed by real-time PCR analysis in the PR-cre; Bmp2fl/fl (termed Bmp2d/d) uterus. While littermate controls average 0.9 litter of 6.2 ± 0.7 pups per month, Bmp2d/d females are completely infertile. Analysis of the infertility indicates that whereas embryo attachment is normal in the Bmp2d/d as in control mice, the uterine stroma is incapable of undergoing the decidual reaction to support further embryonic development. Recombinant human BMP2 can partially rescue the decidual response, suggesting that the observed phenotypes are not due to a developmental consequence of Bmp2 ablation. Microarray analysis demonstrates that ablation of Bmp2 leads to specific gene changes, including disruption of the Wnt signaling pathway, Progesterone receptor (PR) signaling, and the induction of prostaglandin synthase 2 (Ptgs2). Taken together, these data demonstrate that Bmp2 is a critical regulator of gene expression and function in the murine uterus.
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| Contributor(s) |
Lee KY, Jeong J, Wang J, Ma L, Martin JF, Tsai SY, Lydon JP, DeMayo FJ |
| Citation(s) |
17515606 |
| Submission date |
Mar 20, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Michael J Large |
| E-mail(s) |
large@bcm.edu
|
| Phone |
713-798-6980
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular & Cellular Biology
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| Lab |
DeMayo
|
| Street address |
One Baylor Plaza
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| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM276694 |
decidualizing Bmp2 f/f uterus horn-1 |
| GSM276695 |
decidualizing Bmp2 f/f uterus horn-2 |
| GSM276696 |
decidualizing Bmp2 f/f uterus horn-3 |
| GSM276697 |
decidualizing Bmp2 d/d uterus horn-1 |
| GSM276698 |
decidualizing Bmp2 d/d uterus horn-2 |
| GSM276699 |
decidualizing Bmp2 d/d uterus horn-3 |
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| Relations |
| BioProject |
PRJNA107209 |
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