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Series GSE109061 Query DataSets for GSE109061
Status Public on Aug 21, 2018
Title A novel transcriptional network for the Androgen Receptor in human epididymis epithelial cells [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The androgen receptor (AR) has a pivotal role in regulating gene expression in the male reproductive system. Due to the involvement of AR in prostate cancer, its role is best studied in the prostate gland epithelium and prostate cancer cell lines. Here we investigate the transcriptional program of AR in normal human epididymis epithelial (HEE) cells. After AR stimulation of caput HEE cells with the synthetic androgen R1881, AR targets were revealed with RNA-sequencing. Next, AR occupancy genome-wide was determined in control or R1881-stimulated HEE cells by chromatin immunoprecipitation and deep sequencing (ChIP-seq). The results identify about 200 genes that are differentially expressed (DEGs) in HEE cells after AR activation. Some of these DEGs show occupancy of AR at their promoters or cis-regulatory elements suggesting direct regulation. However there is little overlap in AR-associated DEGs between HEE and prostate epithelial cells. Inspection of over-represented motifs in AR ChIP-seq peaks identified CAAT-enhancer binding protein beta (CEBPB) and Runt-related transcription factor 1 (RUNX1) as potential co-factors, with no evidence for FOXA1, which is an important co-factor in the prostate epithelium. CEBPB and RUNX1 ChIP-seq in HEE cells showed that both these factors often occupied AR-binding sites, though rarely simultaneously. Further analysis at a single AR-regulated locus (FK506-binding protein 5, FKPB5) suggests that RUNX1 may inhibit AR occupancy, while CEBP appears to be a co-activator. These data suggest a novel AR transcriptional network governs differentiated functions of the human epididymis epithelium.
Overall design Examination of AR cistrome in primary human epididymis cells with or without R1881 treatment
Contributor(s) Yang R, Browne JA, Eggener SE, Leir S, Harris A
Citation(s) 30016502
Submission date Jan 11, 2018
Last update date Mar 27, 2019
Contact name Ann Harris
Organization name Case Western Reserve Uni
Street address 10900 Euclid Ave
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (13)
GSM2929022 input_DNA
GSM2929023 AR_VC_rep1
GSM2929024 AR_R1881_rep1
This SubSeries is part of SuperSeries:
GSE109063 A novel transcriptional network for the Androgen Receptor in human epididymis epithelial cells
BioProject PRJNA429558
SRA SRP130958

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Supplementary file Size Download File type/resource
GSE109061_RAW.tar 715.0 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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