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Series GSE108883 Query DataSets for GSE108883
Status Public on Jan 09, 2018
Title Role of SUMOylation in differential ERα transcriptional repression by SERMs and pure antiestrogens in breast cancer cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary RNA-seq: Gene expression profiling in MCF-7 cells treated with vehicle (0), estradiol (E2), the Selective ER Modulator 4-hydroxytamoxifen (OHT), or the pure antiestrogen fulvestrant (ICI). ChIP-seq: Genome-wide DNA binding profile of ERα and SUMO2/3 in MCF-7 cells treated with vehicle, E2 or ICI.
 
Overall design RNA-seq: MCF-7 cells cultured in estrogen-depleted media were treated with vehicle, 5 nM E2, 100 nM OHT or 100 nM ICI for 16 h. Fold-Change of mRNA levels compared to vehicle was determined for each treatment condition (3 biological replicates for each). ChIP-seq: MCF-7 cells cultured in estrogen-depleted media were treated with vehicle, 5 nM E2 or 100 nM ICI for 30 min or 3 h. ChIP-seq with an antibody for ERα (Santa Cruz Biotechnology sc-543) was performed, and ERα peaks were called for each condition (3 biological replicates) relative to the corresponding input samples. MCF-7 cells cultured in estrogen-depleted media were treated with vehicle or 100 nM ICI for 30 min or 3 h. ChIP-seq with an antibody for SUMO2/3 (Cedarlane M114-3) was performed, and SUMO2/3 peaks were called for each condition (2 biological replicates) relative to the corresponding input samples.
 
Contributor(s) Traboulsi T, Dumeaux V, Audemard E, Mader S
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Submission date Jan 08, 2018
Last update date Mar 26, 2019
Contact name Sylvie Mader
E-mail(s) sylvie.mader@umontreal.ca
Phone 514 3437166
Organization name Université de Montréal
Department Biochemistry and Molecular Medicine
Lab Molecular targeting in breast cancer
Street address CP 6128 Succursale centre ville
City MONTREAL
State/province QC
ZIP/Postal code H3C 3J7
Country Canada
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (40)
GSM2915375 DSP250_EtOH_ERa
GSM2915376 DSP488_V_30_ERa_N1
GSM2915377 DSP514_V_30_ERa_N1
Relations
BioProject PRJNA429003
SRA SRP128564

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108883_RAW.tar 62.1 Mb (http)(custom) TAR (of TSV, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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