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| Status |
Public on Jan 09, 2018 |
| Title |
Role of SUMOylation in differential ERα transcriptional repression by SERMs and pure antiestrogens in breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
RNA-seq: Gene expression profiling in MCF-7 cells treated with vehicle (0), estradiol (E2), the Selective ER Modulator 4-hydroxytamoxifen (OHT), or the pure antiestrogen fulvestrant (ICI). ChIP-seq: Genome-wide DNA binding profile of ERα and SUMO2/3 in MCF-7 cells treated with vehicle, E2 or ICI.
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| Overall design |
RNA-seq: MCF-7 cells cultured in estrogen-depleted media were treated with vehicle, 5 nM E2, 100 nM OHT or 100 nM ICI for 16 h. Fold-Change of mRNA levels compared to vehicle was determined for each treatment condition (3 biological replicates for each). ChIP-seq: MCF-7 cells cultured in estrogen-depleted media were treated with vehicle, 5 nM E2 or 100 nM ICI for 30 min or 3 h. ChIP-seq with an antibody for ERα (Santa Cruz Biotechnology sc-543) was performed, and ERα peaks were called for each condition (3 biological replicates) relative to the corresponding input samples. MCF-7 cells cultured in estrogen-depleted media were treated with vehicle or 100 nM ICI for 30 min or 3 h. ChIP-seq with an antibody for SUMO2/3 (Cedarlane M114-3) was performed, and SUMO2/3 peaks were called for each condition (2 biological replicates) relative to the corresponding input samples.
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| Contributor(s) |
Traboulsi T, Dumeaux V, Audemard E, Mader S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 08, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Sylvie Mader |
| E-mail(s) |
sylvie.mader@umontreal.ca
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| Phone |
514 3437166
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| Organization name |
Université de Montréal
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| Department |
Biochemistry and Molecular Medicine
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| Lab |
Molecular targeting in breast cancer
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| Street address |
CP 6128 Succursale centre ville
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| City |
MONTREAL |
| State/province |
QC |
| ZIP/Postal code |
H3C 3J7 |
| Country |
Canada |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA429003 |
| SRA |
SRP128564 |
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