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Series GSE108796 Query DataSets for GSE108796
Status Public on Jan 05, 2021
Title Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Objectives: To characterize the transcripts profile of PBMCs when co-cultured with HCC cell lines using High-Throughput Sequencing and To validate the differentially expressed genes via quantitative reverse transcription polymerase chain reaction (qRT-PCR) in PBMCs of HCC pateints.
Methods: Transcription profiles of PBMC (control) and PBMC co-cultured with liver cancer cell lines (treatment) were generated by deep sequencing, in triplicate, using Illumina Illumina HiSeq 4000.
Results and conclusion: Transcriptome analysis of PBMCs has revealed the presence of biological process alterations when co-cultured with HCC cell lines and qRT-PCR validation have indicated that four genes were up-reglurated in HCC patients when compared with healthy donors including IL1b, INHBA, PLOD2, PRG4.
 
Overall design Transcription profiles of PBMC (control) and PBMC co-cultured with cancer (treatment) were generated by deep sequencing, using Illumina HiSeq 4000.
 
Contributor(s) Kunadirek P, Chuaypen N, Tangkijvanich P
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Submission date Jan 05, 2018
Last update date Jan 05, 2021
Contact name Natthaya Chuaypen
E-mail(s) nchuaypen@gmail.com
Organization name Chulalongkorn University
Street address 1873 Rama 4 road, Pathumwan
City Bangkok
ZIP/Postal code 10330
Country Thailand
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (9)
GSM2913325 PBMCs from Healthy 1
GSM2913326 PBMCs from Healthy 2
GSM2913327 PBMCs from Healthy 3
Relations
BioProject PRJNA428610
SRA SRP128096

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108796_Controls_vs_Treatments_HepG2.txt.gz 429.0 Kb (ftp)(http) TXT
GSE108796_Controls_vs_Treatments_Huh7.txt.gz 451.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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