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| Status |
Public on Jan 05, 2021 |
| Title |
Analysis of gene expression in co-culture of PBMCs with Hepatocellular carcinoma by High-Throughput Sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Objectives: To characterize the transcripts profile of PBMCs when co-cultured with HCC cell lines using High-Throughput Sequencing and To validate the differentially expressed genes via quantitative reverse transcription polymerase chain reaction (qRT-PCR) in PBMCs of HCC pateints.
Methods: Transcription profiles of PBMC (control) and PBMC co-cultured with liver cancer cell lines (treatment) were generated by deep sequencing, in triplicate, using Illumina Illumina HiSeq 4000.
Results and conclusion: Transcriptome analysis of PBMCs has revealed the presence of biological process alterations when co-cultured with HCC cell lines and qRT-PCR validation have indicated that four genes were up-reglurated in HCC patients when compared with healthy donors including IL1b, INHBA, PLOD2, PRG4.
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| Overall design |
Transcription profiles of PBMC (control) and PBMC co-cultured with cancer (treatment) were generated by deep sequencing, using Illumina HiSeq 4000.
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| Contributor(s) |
Kunadirek P, Chuaypen N, Tangkijvanich P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 05, 2018 |
| Last update date |
Jan 05, 2021 |
| Contact name |
Natthaya Chuaypen |
| E-mail(s) |
nchuaypen@gmail.com
|
| Organization name |
Chulalongkorn University
|
| Street address |
1873 Rama 4 road, Pathumwan
|
| City |
Bangkok |
| ZIP/Postal code |
10330 |
| Country |
Thailand |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (9)
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| GSM2913328 |
PBMCs from Healthy 1 co-cultured with HepG2 |
| GSM2913329 |
PBMCs from Healthy 2 co-cultured with HepG2 |
| GSM2913330 |
PBMCs from Healthy 3 co-cultured with HepG2 |
| GSM2913331 |
PBMCs from Healthy 1 co-cultured with Huh7 |
| GSM2913332 |
PBMCs from Healthy 2 co-cultured with Huh7 |
| GSM2913333 |
PBMCs from Healthy 3 co-cultured with Huh7 |
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| Relations |
| BioProject |
PRJNA428610 |
| SRA |
SRP128096 |
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