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| Status |
Public on Jan 04, 2021 |
| Title |
H3K4me3/H3K27me3 bivalent chromatin function to fine-tune gene expression level |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Bivalency, the paradoxical juxtaposition of transcriptionally activating trimethylation of histone H3 lysine 4 (H3K4me3) and the repressive trimethylation of histone H3 lysine 27 (H3K27me3), has been proposed to decorate developmental genes poised for gene expression regulation. Here, we report development of sequential internally calibrated chromatin immunoprecipitation (Re-ICeChIP-seq), capable of measuring absolute quantities of nucleosomal patterns of histone marks in a genome-wide fashion, combined with in situ control of antibody specificity. Re-ICeChIP-seq of H3K4me3/H3K27me3 in mESC reveals that bivalent genes can be delineated into two classes, distinguished by the nucleosomal ratio of H3K4me3 to H3K27me3. Consistent with the canonical role of bivalency, H3K27me3-rich bivalent nucleosomes demarcate promoters of poorly expressed developmental genes that may be poised for activation or repression. Yet our measurements reveal surprisingly widespread presence of bivalency at promoters of highly-expressed housekeeping genes, characterized by H3K4me3-rich bivalent nucleosomes. Moreover, the ratio of H3K4me3 to H3K27me3 at transcription start sites better correlates with gene expression than H3K4me3 or H3K27me3 alone, suggesting cooperation between opposing marks to fine-tune gene expression. Finally, we report that major H3K4 methyltransferases exhibit wide acceptance of various H3K27me3 substrates.
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| Overall design |
Examination of H3K4me3/H3K27me3 bivalency in mouse Embryonic Stem Cells using Sequential ICeChIP-seq.
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| Contributor(s) |
Grzybowski AT, Chen Z, Lechner CC, Hattorie T, Fierz B, Koide S, Ruthenburg AJ |
| Citation missing |
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| |
| Submission date |
Jan 04, 2018 |
| Last update date |
Jan 06, 2021 |
| Contact name |
Alexander Ruthenburg |
| Organization name |
The University of Chicago
|
| Department |
Molecular Genetics and Cell Biology
|
| Lab |
Ruthenburg Laboratory of Chromatin Biochemistry & Structure
|
| Street address |
920 E 58th St
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA428482 |
| SRA |
SRP128041 |
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