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Series GSE108736 Query DataSets for GSE108736
Status Public on Jan 11, 2018
Title Defining the Essential Function of Yeast Hsf1 Reveals a Compact Transcriptional Program for Maintaining Eukaryotic Proteostasis
Organisms Saccharomyces cerevisiae; Mus musculus
Experiment type Other
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We used the conditional chemical genetics approach known as “anchor away” (AA) to rapidly inactivate the essential yeast transcription factor Hsf1. We coupled Hsf1-AA to RNA-seq and NET-seq to define the genes whose expression depends on Hsf1 and performed Hsf1-3xFLAG-V5 ChIP-seq to validate direct targets. We also carried out a number of other perturbations to yeast stress pathways to show that most of the gene expression changes during heat shock are Hsf1-independent but depend on PKA signaling and the Msn2/4 general stress transcription factors. Finally, we generated RNA-seq in mouse ES cells and MEFs in wild type and hsf1-/- cells to define HSF1 targets in murine cells.
Overall design yeast RNA-seq samples, 4 yeast NET-seq samples, 8 murine RNA seq samples and 1 yeast ChIP-seq sample
Contributor(s) Pincus D, Denic V
Citation(s) 27320198, 29395071
NIH grant(s)
Grant ID Grant title Affiliation Name
DP5 OD017941 Quantitative approaches to reveal the homeostatic control mechanisms of stress re WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH David Pincus
Submission date Jan 04, 2018
Last update date Nov 10, 2020
Contact name David Pincus
Phone 7737950313
Organization name University of Chicago
Street address 929 E 56th St.
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (38)
GSM2912699 Hsf1-AA_NET_0_1
GSM2912700 Hsf1-AA_NET_0_2
GSM2912701 Hsf1-AA_NET_0_3
BioProject PRJNA428450
SRA SRP128029

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108736_ChIP-seq.txt.gz 1.8 Kb (ftp)(http) TXT
GSE108736_Hsf1-AA_RNA_C_R_H_RH.txt.gz 101.5 Kb (ftp)(http) TXT
GSE108736_Hsf1-AA_RNA_R_tc.txt.gz 99.6 Kb (ftp)(http) TXT
GSE108736_MEF_RNA.txt.gz 344.1 Kb (ftp)(http) TXT
GSE108736_NET-seq_data.txt.gz 781.6 Kb (ftp)(http) TXT
GSE108736_mESC_RNA.txt.gz 467.0 Kb (ftp)(http) TXT
GSE108736_msn24_RNA_C_HS_1NM.txt.gz 79.1 Kb (ftp)(http) TXT
GSE108736_tpk123_RNA_C_HS_1NM.txt.gz 79.3 Kb (ftp)(http) TXT
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