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Series GSE108704 Query DataSets for GSE108704
Status Public on Jan 01, 2019
Title lncRNA LINC00844 regulates prostate cancer cell migration and invasion through AR signaling [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The majority of the human genome is transcribed, yielding a rich repository of non-coding transcripts that are involved in a myriad of biological processes including cancer. However, how non-coding transcripts such as Long Non-coding RNAs (lncRNAs) function in prostate cancer is still unclear. In this study, we have identified a novel set of clinically relevant androgen-regulated lncRNAs in prostate cancer. Among this group, we found LINC00844 is a direct androgen regulated target that is actively transcribed in AR-dependent prostate cancer cells. In clinical analysis, the expression of LINC00844 is higher in normal prostate compared to malignant and metastatic prostate cancer samples and patients with low expression demonstrate poor prognosis and significantly increased biochemical recurrence suggesting LINC00844 may function in suppressing tumor progression and metastasis. From in-vitro loss-of-function studies, we showed LINC00844 prevents prostate cancer cell migration and invasion. Moreover, in gene expression studies we demonstrate LINC00844 functions in trans, affecting global androgen-regulated gene transcription. Mechanistically, we provide evidence to show LINC00844 is important in facilitating AR binding to the chromatin. Finally, we showed LINC00844 mediates its phenotypic effects in part by activating the expression of NDRG1, a crucial cancer metastasis suppressor. Collectively, our findings indicate LINC00844 is a novel coregulator of AR that plays an important role in the androgen transcriptional network and the development and progression of prostate cancer.
Overall design AR ChIP-seq in LNCaP cells treated with siControl (NC) or siLINC00844#1 and #4 and stimulated with 100nM of DHT or vehicle control for 2 hours. All the experiments are performed in duplicates.
Contributor(s) Cheung E
Citation(s) 30115758
BioProject PRJNA420439
Submission date Jan 03, 2018
Last update date Apr 03, 2019
Contact name Edwin Cheung
Phone +58362720097
Organization name University of Macau
Department Faculty of Health Sciences
Lab Edwin Cheung Lab
Street address Taipa
City Macau
State/province Macau
ZIP/Postal code 999078
Country Macau
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (12)
GSM2911384 NC_DHT_R1
GSM2911385 NC_DHT_R2
GSM2911386 NC_ETOH_R1
SRA SRP125916

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Supplementary file Size Download File type/resource
GSE108704_RAW.tar 1.2 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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