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| Status |
Public on Oct 22, 2019 |
| Title |
GR cistromes reprogramming by High Fat Diet [ChIP-seq: GR day/night] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We mapped the genome-wide binding profiles of GR throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks
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| Overall design |
C57BL/6J mice were fed High Fat Diet (Research Diet, D12331) and control diet (Research Diet, D12329) for 12 weeks. Mice were sacrificed by cervical dislocation every 4 hours along the day, liver collected and snap frozen. ChIP was performed as previously described (Uhlenhaut et al. 2012). Sheared chromatin was immunoprecipitated with anti-GR antibody (Santa Cruz, sc-1004X), anti-PPARalpha antibody (Santa Cruz, sc-9000), and a mixture of anti-Stat5a/b (RD System, AF2168) and anti-Stat5a/b (pY694/Y699) (Cell Signaling, 9351). Two replicates per condition and time point were used, for a total of 24 GR (ZT0-ZT20, control/HFD), 4 PPAR alpha (ZT12, control/HFD), and 4 STAT5 (ZT12, control/HFD) ChIP-seq samples.
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| Contributor(s) |
Mir AA, Quagliarini F |
| Citation(s) |
31706703 |
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| Submission date |
Jan 03, 2018 |
| Last update date |
Jan 21, 2020 |
| Contact name |
Nina Henriette Uhlenhaut |
| E-mail(s) |
henriette.uhlenhaut@helmholtz-muenchen.de
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| Organization name |
HelmholtzZentrum München - IDC
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| Department |
Molecular Endocrinology
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| Lab |
Uhlenhaut IDC
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| Street address |
Ingolstädter Landstraße 1, Neuherberg
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
D-85764 |
| Country |
Germany |
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| Platforms (3) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (34)
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| This SubSeries is part of SuperSeries: |
| GSE108690 |
Cistromic reprogramming of the diurnal glucocorticoid hormone response by high fat diet |
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| Relations |
| BioProject |
PRJNA428305 |
| SRA |
SRP130926 |
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