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Series GSE108600 Query DataSets for GSE108600
Status Public on Jul 11, 2018
Title CUT&RUN chromatin profiling of endogenously tagged and native BATF
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Primary human CD4+ T cells were isolated and an N-terminal GFP tag was inserted into the endogenous BATF locus. CUT&RUN using anti-GFP or anti-BATF antibodies was performed to assay chromatin occupancy. Libraries were prepared and sequenced on Illumina NextSeq platform.
Overall design CUT&RUN using BATF or GFP antibodies followed by library preparation and 75 nt paired-end sequencing on Illumina NextSeq machines
Contributor(s) Roth TL, Yu R, Marson A
Citation(s) 29995861
Submission date Dec 28, 2017
Last update date Mar 26, 2019
Contact name Ruby Yu
Organization name UCSF
Street address 513 Parnassus Ave
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (10)
GSM2905795 Donor1_noelectroporation_noantibody
GSM2905796 Donor1_noelectroporation_antiBATF
GSM2905797 Donor1_noelectroporation_antiGFP
BioProject PRJNA427801
SRA SRP127664

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Supplementary file Size Download File type/resource
GSE108600_RAW.tar 5.2 Gb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data are available on Series record

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