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Series GSE10847 Query DataSets for GSE10847
Status Public on Mar 18, 2008
Title H522 lung cancer cells' TGFbeta response was restored by putting back BRG1 and TGFbRII
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The transcription factors Smad2 and Smad3 mediate a large set of genes responses induced by the cytokine TGFβ, but the extent to which their function depends on chromatin remodeling remains to be defined. We observed interactions between these two Smads and BRG1, BAF250b, BAF170 and BAF155, which are core components of the SWI/SNF chromatin-remodeling complex. Smad2 and Smad3 have a similar affinity for these components in vitro, and their interactions are primarily mediated by BRG1. In vivo, however, BRG1 predominantly interacts with Smad3, and this interaction is enhanced by TGFβ stimulation. Our results suggest that BRG1 is incorporated into transcriptional complexes that are formed by activated Smads in the nucleus, on target promoters. Using BRG1-deficient cell systems(H522 lung cancer cells), we defined the BRG1 dependence of the TGFβ transcriptional program genome-wide. Most TGFβ gene responses in human epithelial cells are dependent on BRG1 function. Remarkably, BRG1 is not required for the TGFβ-mediated induction of SMAD7 and SNON, which encode key mediators of negative feedback in this pathway. Our results provide a genome-wide scope of the participation of BRG1 in TGFβ action and suggest a widespread yet differential involvement of BRG1 SWI/SNF remodeler in the transcriptional response of many genes to this cytokine.
Keywords: comparative genomic hybridization
 
Overall design NCI-H522 cells that were transduced with BRG1, TβR-II, or both vectors combined were incubated with TGFβ and subjected to transcriptomic analysis. Totally 6 samples.
 
Contributor(s) Xi Q, He W, Zhang XH, Massague J
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Submission date Mar 14, 2008
Last update date Jan 08, 2019
Contact name Qiaoran Xi
E-mail xiq@mskcc.org
Organization name MSKCC
Department Cancer biology and genetics
Lab Joan Massague
Street address 1275, York ave.
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM272100 H522-BRG1
GSM275309 H522-BRG1-TbRII-Tb
GSM275311 H522-BRG1-TbRII
Relations
BioProject PRJNA107337

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10847_RAW.tar 137.6 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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