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Series GSE108291 Query DataSets for GSE108291
Status Public on Jan 09, 2018
Title Single cell RNA-Seq of E18.5 developing mouse kidney and human kidney organoids
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary These files represent single cell RNA-Seq data generated on a 10x Chromium genomics platform from three biological replicates from the embryonic day (E)18.5 developing mouse kidney and three biological replicates of iPSC-derived human kidney organoids differentiated according to our published protocol (Takasato et al., Nature Protocols 2016). When aggregated, the mouse data represents >6000 cells that passed our QC, containing most major cell types known to exist in the developing mouse kidney. The aggregated human organoid data contains of >7000 cells that passed our QC and contains populations representing endothelial cells, podocytes, stroma, nephron, and off-target populations with similarity to neurons.
 
Overall design Examination of the celular composition of human kidney organoids by comparison to mouse embryonic kidney
Web link http://www.biorxiv.org/content/biorxiv/early/2017/12/22/235499.full.pdf
 
Contributor(s) Zappia L, Combes AN
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Dec 19, 2017
Last update date Mar 27, 2019
Contact name Luke Zappia
Organization name Murdoch Childrens Research Institute
Street address 50 Flemington Road
City Parkville
ZIP/Postal code 3052
Country Australia
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (7)
GSM2914994 E18.5 kidney 1
GSM2914995 E18.5 kidney 2
GSM2914996 E18.5 kidney 3
Relations
BioProject PRJNA428938
SRA SRP130947

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108291_kid_barcodes.tsv.gz 6.5 Mb (ftp)(http) TSV
GSE108291_kid_genes.tsv.gz 212.7 Kb (ftp)(http) TSV
GSE108291_kid_matrix.mtx.gz 135.0 Mb (ftp)(http) MTX
GSE108291_kidney_counts.csv.gz 20.2 Mb (ftp)(http) CSV
GSE108291_org4_barcodes.tsv.gz 6.6 Kb (ftp)(http) TSV
GSE108291_org4_counts.csv.gz 5.7 Mb (ftp)(http) CSV
GSE108291_org4_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE108291_org4_matrix.mtx.gz 16.1 Mb (ftp)(http) MTX
GSE108291_org_barcodes.tsv.gz 6.5 Mb (ftp)(http) TSV
GSE108291_org_counts.csv.gz 20.5 Mb (ftp)(http) CSV
GSE108291_org_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE108291_org_matrix.mtx.gz 103.2 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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