|
| Status |
Public on Dec 20, 2017 |
| Title |
RNAs associated with affinity captured LINE-1 ribonucleoproteins |
| Organism |
Homo sapiens |
| Experiment type |
Other
Expression profiling by high throughput sequencing
|
| Summary |
RNAs extracted from affinity enriched L1 RNPs were subjected to next-gen RNA-seq
|
| |
|
| Overall design |
Synthetic L1s expressed in HEK293T_LD from pLD401, pLD567, and pLD624
Synthetic L1s expressed from pLD401 were subjected to split-tandem affinity enrichement, via ORF2p-3xFLAG (1st) and direct IP of ORF1p (2nd)
Synthetic L1s expressed from pLD567 and pLD624 were subjected to affinity enrichement via ORF2p-3xFLAG only
|
| |
|
| Contributor(s) |
LaCava J |
| Citation(s) |
29309035 |
| |
| Submission date |
Dec 19, 2017 |
| Last update date |
Mar 26, 2019 |
| Contact name |
John LaCava |
| E-mail(s) |
jlacava@rockefeller.edu
|
| Organization name |
The Rockefeller University
|
| Street address |
1230 York ave
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA423015 |
| SRA |
SRP127029 |
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