|
| Status |
Public on Oct 05, 2018 |
| Title |
TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In this study, proteomic profiling of TRIM24 interactome in conjunction with shRNA screening of TRIM24 top-interactors nominated that TRIM28 is indispensable for TRIM24 protein stability. We showed that TRIM28 stabilizes TRIM24 against SPOP-mediated ubiquitination and degradation. TRIM28 promotes TRIM24 and AR transcription activity, androgen-dependent and -independent PCa growth. In addition, we demonstrated that TRIM28 level in high in advanced PCa, which drives TRIM24/AR transcription activity in a similar manner to SPOP mutation, which implies that TRIM28 potentially dictates the therapeutic outcome of TRIM24-targeted therapy.
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| |
|
| Overall design |
genetic modification design
|
| |
|
| Contributor(s) |
Yu J, Fong K |
| Citation(s) |
30479348 |
| |
| Submission date |
Dec 15, 2017 |
| Last update date |
Feb 04, 2020 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
|
| Samples (7)
|
|
| This SubSeries is part of SuperSeries: |
| GSE108146 |
TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression |
|
| Relations |
| BioProject |
PRJNA422654 |
| SRA |
SRP126894 |
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