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Series GSE108077 Query DataSets for GSE108077
Status Public on Mar 01, 2018
Title Warming Induces Significant Reprogramming of Beige, but Not Brown, Adipocyte Cellular Identity
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Beige and brown adipocytes generate heat in response to reductions in ambient temperature. When warmed, both beige and brown adipocytes exhibit morphological ‘whitening’, but it is unknown whether or to what extent this represents a true shift in cellular identity. Using cell type-specific profiling in vivo, we uncover a unique paradigm of temperature-dependent epigenomic plasticity of beige, but not brown, adipocytes, with conversion from a brown to a white chromatin state. Despite this profound shift in cellular identity, warm whitened beige adipocytes retain an epigenomic memory of prior cold exposure defined by an array of poised enhancers that prime thermogenic genes for rapid response during a second bout of cold exposure. We further show that a transcriptional cascade involving the glucocorticoid receptor and Zfp423 can drive warm-induced whitening of beige adipocytes. These studies identify the epigenomic and transcriptional bases of an extraordinary example of cellular plasticity in response to environmental signals.
 
Overall design Ucp1-NuTRAP and Adipoq-NuTRAP mice exposed to different temperatures were used for brown, beige or white adipocyte-specific transcriptomic and epigenomic analysis.
 
Contributor(s) Roh H, Rosen E
Citation(s) 29657031
Submission date Dec 14, 2017
Last update date Mar 25, 2019
Contact name Evan Rosen
E-mail(s) erosen@bidmc.harvard.edu
Organization name Beth Israel Deconess Medical Center
Department Endocrinology
Lab Rosen Lab
Street address 3 Blackfan Cir
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (58)
GSM2889114 Cold brown adipocyte mRNA rep 1
GSM2889115 Cold brown adipocyte mRNA rep 2
GSM2889116 Cold brown adipocyte mRNA rep 3
Relations
BioProject PRJNA422411
SRA SRP126748

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108077_RAW.tar 3.8 Gb (http)(custom) TAR (of BW)
GSE108077_mRNA_cpms_with_batch.tsv.gz 517.3 Kb (ftp)(http) TSV
SRA Run SelectorHelp
Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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