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Series GSE108045 Query DataSets for GSE108045
Status Public on Dec 14, 2017
Title Genome wide study of adult microglia in Specific-pathogen-free (SPF) mice treated or not during one week with antibiotics (ABX) in males and females.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNAseq was performed on microglia from male and female, controls and ABX to elucidate the genetic differences implicates by microbiota and gender.
Overall design Microglia from SPF and GF mice from embryonic and adult stages of both gender were sorted for sequencing. DEGs were obtained to observe if any significant genes were affected. Pathway analysis was performed with the set of DEGs. SPF mice were given ampicillin (1mg/ml), streptomycin (5mg/ml), colistin (1mg/ml) and amphotericin (0.1mg/ml) in sterile drinking water ad libitum for one week, between 7 and 8 weeks of age. Sterile drinking water devoid of antibiotics was provided to control mice ad libitum.
Contributor(s) Thion MS, Coulpier F, Garel S
Citation(s) 29275859
Submission date Dec 13, 2017
Last update date May 15, 2019
Contact name jinmiao chen
Organization name Singapore Immunology Network
Street address 8A Biomedical Grove, #04-06 Immunos
City Singapore
ZIP/Postal code 138648
Country Singapore
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2888397 Adult SPF Female Replicate 1
GSM2888398 Adult SPF Female Replicate 2
GSM2888399 Adult SPF Female Replicate 3
This SubSeries is part of SuperSeries:
GSE107130 Microbiome influences prenatal and adult microglia in a sex-specific
BioProject PRJNA422281
SRA SRP126679

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108045_readCount_geneName_exp2.txt.gz 3.2 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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