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Series GSE107873 Query DataSets for GSE107873
Status Public on Jun 14, 2018
Title Tetraspanin family member functionally resolves and facilitates the purification of adult pluripotent stem cells used for whole-body regeneration (Single)
Organism Schmidtea mediterranea
Experiment type Expression profiling by high throughput sequencing
Summary Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) capable of sustaining tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single cell transplantation to address this long-standing issue. Large-scale scRNA-seq of fluorescently sorted neoblasts unveiled a novel subtype of neoblast (Nb2) characterized by high levels of PIWI-1 mRNA and protein, and marked by a conserved cell-surface protein coding gene, tetraspanin 1 (tspan1). tspan1-positive cells survived sub-lethal irradiation, underwent clonal expansion to repopulate whole animals, and when purified with an anti-TSPAN-1 antibody rescued with high efficiency the viability of lethally irradiated animals after single-cell transplantation. Our work demonstrates the first prospective isolation of an adult PSC, bridges a conceptual dichotomy between operationally and molecularly defined neoblasts, and sheds light on the mechanisms governing in vivo pluripotency and the source of regeneration in planarians.
Overall design 1) Examine gene expression profiles of sorted 80 X1 and 80 Xins cell at single cell level using the plate based single cell RNA-seq platform. Two batch of (40 X1+40 Xins) cells were sorted into individual 96-well plates, and libraries were made by ClonTech v4 full reactions, and quarter-size reactions, respectively. 2) Examine gene expression profiles of sorted X1 cells, and X1+X2 cells survived in 7-day irradiation by 10X scRNA-Seq platform.
Contributor(s) Zeng A, Li H, Sánchez Alvarado A
Citation(s) 29906446
Submission date Dec 08, 2017
Last update date May 15, 2019
Contact name Hua Li
Organization name Stowers Institute for Medical Research
Street address 1000 E 50th Street
City Kansas City
State/province MO
ZIP/Postal code 64110
Country USA
Platforms (1)
GPL20150 Illumina HiSeq 2500 (Schmidtea mediterranea)
Samples (162)
GSM2882418 ClonTechv4_Xins-cell-39
GSM2882419 ClonTechv4_Xins-cell-38
GSM2882420 ClonTechv4_Xins-cell-36
This SubSeries is part of SuperSeries:
GSE107875 A tetraspanin family member functionally resolves and facilitates the purification of adult pluripotent stem cells used for whole-body regeneration
BioProject PRJNA421769
SRA SRP126427

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE107873_ClonTechv4_read_counts.xlsx 10.9 Mb (ftp)(http) XLSX
GSE107873_Mantis_read_counts.xlsx 11.0 Mb (ftp)(http) XLSX
GSE107873_Sublethal_cells_10x_exprs.csv.gz 9.4 Mb (ftp)(http) CSV
GSE107873_X1_cells_10x_exprs.csv.gz 74.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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