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| Status |
Public on May 26, 2021 |
| Title |
RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes after Twist1 knockout in mouse bone marrow stromal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to determine possible downstream targets of Twist1 in bone marrow stromal cells with or without Twist1 knockout.
Methods: gene expression profiling with or without Twist1 knockout in bone marrow stromal cells were generated by deep sequencing, using Illumina Hiseq.The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays.
Conclusions: Notch signaling were activated after Twist1 knockout.
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| Overall design |
mRNA profiles of stromal cells with or without Twist1 knockout were generated by deep sequencing using Illumina Hiseq.
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| Contributor(s) |
Ma X, Liu X |
| Citation(s) |
30026340 |
| |
| Submission date |
Dec 07, 2017 |
| Last update date |
Aug 25, 2021 |
| Contact name |
Xiao Tong Ma |
| E-mail(s) |
maxt@ihcams.ac.cn
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| Phone |
+862223909405
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| Organization name |
Chinese Academy of Medical Sciences
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| Department |
Institute of Hematology & Blood Diseases Hospital
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| Lab |
State Key Laboratory of Experimental Hematology
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| Street address |
Blood hospital, 288 Nanjing Road
|
| City |
TianJin |
| State/province |
TianJin |
| ZIP/Postal code |
300020 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA421494 |
| SRA |
SRP126346 |
