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Series GSE10778 Query DataSets for GSE10778
Status Public on Jun 30, 2009
Title Comparison of VEGF versus EGF gene expression in HUVEC
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Angiogenesis is defined as the formation of new capillaries by sprouting from preexisting vessels. It is mainly triggered by vascular endothelial growth factor (VEGF) and occurs in the adult primarily in wound healing processes or in pathologic tumor vessel growth. To identify genes specifically triggered by VEGF and involved in the process of angiogenesis, we utilized Affymetrix microarrays hybridized with cRNA of human umbilical vein endothelial cells (HUVEC) stimulated with either the main trigger of angiogenesis, VEGF or a more general mitogenic growth factor, EGF.
This was done under the assumption that, in comparison to EGF, VEGF would induce an additional set of genes not solely required for cell division but necessary for angiogenesis. The obtained data revealed that in HUVEC VEGF induces a large repertoire of genes, the majority of which are not or significantly less induced by EGF.
Keywords: time course
Overall design HUVEC, quiescent by maintaining dense cultures in complete medium for four days without change of medium were stimulted with 100ng of VEGF or 50ng of EGF for 30, 60, 150 and 360 minutes and total RNA was isolated using Trizol.
Contributor(s) Schweighofer B, Bilban M, Hofer E
Citation(s) 19718476
Submission date Mar 11, 2008
Last update date Aug 10, 2018
Contact name Bernhard Schweighofer
Organization name Medical University of Vienna
Department Department of Dermatology
Street address Lazarettgasse 14
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL97 [HG-U133B] Affymetrix Human Genome U133B Array
Samples (14)
GSM272113 HUVEC_ctrl_ChipA
GSM272114 HUVEC_VEGF_30min_ChipA
GSM272115 HUVEC_VEGF_60min_ChipA
BioProject PRJNA107459

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Supplementary file Size Download File type/resource
GSE10778_RAW.tar 137.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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