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| Status |
Public on Dec 05, 2018 |
| Title |
DNA accessibility in CD8+ tumour-infiltrating T cells treated with Phenelzine. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Phenelzine treatments were conducted in tumour-bearing Balb/c mice and then 4T1 tumours were acquired. CD8+ tumour-infiltrating T cells were positively selected by using mouse CD8a (Ly-2) microbeads and isolated by autoMACS Separator with recommended programs. Then isolated CD8 T cells were stimulated by PMA and Ionomycin. Global ATAC-seq analysis was performed to identify the dynamic changes in chromatin accessibility of tumour-filtrating CD8 T cells after phenelzine treatment.
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| Overall design |
4 individual samples, no replicates.
|
| |
|
| Contributor(s) |
Tu WJ, Tan A, Hardy K, Rao S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA421194 |
| SRA |
SRP126186 |
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