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| Status |
Public on Feb 12, 2018 |
| Title |
Role of SKN-1 in dpy-7 and osmotic gene induction |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the use of mRNA sequencing to analyze the role of SKN-1 in regulation of genes under two conditions, dpy-7 mutants and high osmolarity.
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| Overall design |
Total RNA was prepared from gravid young adult Caenorhabditis elegans with three biological replicates and sent to the Yale Center for Genome Analysis for 75 nucleotide single-end sequencing in an Illumina HiSeq 2000. Sequence files were processed with Kallisto to quantify transcripts and Sleuth for differential adundance analysis.
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| |
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| Contributor(s) |
Choe KP, Wu C |
| Citation(s) |
29487136 |
| |
| Submission date |
Dec 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Keith Choe |
| E-mail(s) |
kchoe@ufl.edu
|
| Phone |
3522730139
|
| Organization name |
University of Florida
|
| Street address |
876 Newell Dr, 321 Bartram Hall
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32611 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA421159 |
| SRA |
SRP126163 |
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