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| Status |
Public on Apr 09, 2018 |
| Title |
HC2091 kills Mycobacterium tuberculosis by targeting the MmpL3 mycolic acid transporter |
| Organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The purpose of this study was to characterize the mechanism of action of the small molecule HC2091
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| |
|
| Overall design |
Mtb strain CDC1551 was grown at 37ºC in T-25 vented, standing tissue culture flasks in 8 mL of buffered 7H9 medium to an OD600 of 0.1. Cultures were subsequently treated with 10 µM HC2091 ro SQ109. For the control, cultures treated with an equal amount of DMSO. Following a 24 h incubation period, total bacterial RNA was extracted, quality controlled and sequenced. Each treatment was repeated with two independent (biological) replicates.
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| |
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| Contributor(s) |
Zheng H, Williams JT, Coulson GB, Haiderer ER, Abramovitch RB |
| Citation(s) |
29661875 |
| |
| Submission date |
Nov 29, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Robert B. Abramovitch |
| Organization name |
Michigan State University
|
| Department |
Microbiology and Molecular Genetics
|
| Street address |
567 Wilson Road
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17879 |
Illumina HiSeq 2500 (Mycobacterium tuberculosis) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA420343 |
| SRA |
SRP125816 |
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