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Series GSE107485 Query DataSets for GSE107485
Status Public on Apr 09, 2018
Title HC2091 kills Mycobacterium tuberculosis by targeting the MmpL3 mycolic acid transporter
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by high throughput sequencing
Summary The purpose of this study was to characterize the mechanism of action of the small molecule HC2091
Overall design Mtb strain CDC1551 was grown at 37ºC in T-25 vented, standing tissue culture flasks in 8 mL of buffered 7H9 medium to an OD600 of 0.1. Cultures were subsequently treated with 10 µM HC2091 ro SQ109. For the control, cultures treated with an equal amount of DMSO. Following a 24 h incubation period, total bacterial RNA was extracted, quality controlled and sequenced. Each treatment was repeated with two independent (biological) replicates.
Contributor(s) Zheng H, Williams JT, Coulson GB, Haiderer ER, Abramovitch RB
Citation(s) 29661875
Submission date Nov 29, 2017
Last update date Sep 06, 2018
Contact name Robert B. Abramovitch
Organization name Michigan State University
Department Microbiology and Molecular Genetics
Street address 567 Wilson Road
City East Lansing
State/province MI
ZIP/Postal code 48824
Country USA
Platforms (1)
GPL17879 Illumina HiSeq 2500 (Mycobacterium tuberculosis)
Samples (6)
GSM2868230 WT treated with DMSO, rep1
GSM2868231 WT treated with DMSO, rep2
GSM2868232 WT treated with HC2091, rep1
BioProject PRJNA420343
SRA SRP125816

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Supplementary file Size Download File type/resource
GSE107485_Supplementary_Database_1.xlsx 79.7 Kb (ftp)(http) XLSX
GSE107485_Supplementary_Database_2.xlsx 1.1 Mb (ftp)(http) XLSX
Raw data provided as supplementary file
Processed data is available on Series record

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