|
| Status |
Public on May 15, 2018 |
| Title |
Next Generation Sequencing for Quantitative Transcriptomic Profiling of TLR3, TLR7 and TLR8 Activation in Neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study is to compare gene expression of mouse cultured cortical and hippocampal neurons in response to TLR3, TLR7 and TLR8 activation
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| |
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| Overall design |
At 4 day in vitro, wild-type mouse cortical and hippocampal neurons were treated with vehicle control, poly IC (for TLR3 activation) and CL075 (for TLR7 activation). Tlr7-/- neurons were treated with vehicle control and CL075/poly dT (for TLR8 activation). Six hr after treatment, total RNAs were extracted for deep sequencing, in triplicate, using the NextSeq500 High-output v2 (75 cycle) sequencing kit on the Illumina NextSeq500 instrument.
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| |
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| Contributor(s) |
Hung Y, Chen C, Hsueh Y |
| Citation(s) |
29777026, 29885943 |
| |
| Submission date |
Nov 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yi-Ping Hsueh |
| E-mail(s) |
yph@gate.sinica.edu.tw
|
| Phone |
886-2-27899311
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Molecular Biology
|
| Lab |
N514
|
| Street address |
128 Academia Road, Section 2, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (15)
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|
| Relations |
| BioProject |
PRJNA419291 |
| SRA |
SRP125379 |
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