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Series GSE10719 Query DataSets for GSE10719
Status Public on Jun 02, 2008
Title Response of Arabidopsis cell culture to phytoprostane A1
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary 12-Oxo-phytodienoic acid (OPDA) and several phytoprostanes are structurally related cyclopentenone oxylipins that can be formed via the enzymatic jasmonate pathway and a non-enzymatic, free radical-catalyzed pathway, respectively. To elucidate the biological activities of phytoprostanes in comparison to OPDA as well as the metabolism we performed genome-wide expression analysis.
Keywords: mixotrophic Arabidopsis cell culture, ATH1 Chip
 
Overall design In order to get a comprehensive view of the biological activity of non-enzymatically formed reactive oxylipins the regulation of gene expression in Arabidopsis thaliana was analyzed using the ATH1 chip representing 22500 genes. A mixotrophic cell culture of Arabidopsis was treated with 75 ┬ÁM phytoprostane A1 or with 0.5% methanol in water (Control) and harvested after 4 h. Three independent replicates of each treatment were analyzed and the fold change of normalized signals from PPA1-treated cell culture versus control cell culture was calculated.
 
Citation(s) 18334669
Submission date Mar 04, 2008
Last update date Aug 28, 2018
Contact name Susanne Berger
E-mail(s) berger@biozentrum.uni-wuerzburg.de
Phone 49 931 318 6170
Organization name Julius-von-Sachs-Institute
Department Pharmaceutical Biology
Street address Julius-von-Sachs-Platz 2
City Wuerzburg
ZIP/Postal code 97082
Country Germany
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM270814 Arabidopsis cell culture, 4 h_control_rep1
GSM270853 Arabidopsis cell culture, 4 h_control_rep2
GSM270865 Arabidopsis cell culture, 4 h_control_rep3
This SubSeries is part of SuperSeries:
GSE10749 Response of Arabidopsis cell culture to cyclopentenone oxylipins
Relations
BioProject PRJNA108855

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10719_RAW.tar 13.6 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

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