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Series GSE10708 Query DataSets for GSE10708
Status Public on Mar 15, 2008
Title The orthologue of the RNA chaperone Hfq is essential for cell motility of the cyanobacterium Synechocystis PCC 6803
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary The gene ssr3341 was previously suggested to encode an orthologue of the RNA chaperone Hfq in Synechocystis sp. strain PCC 6803. When a phototactic strain of this cyanobacterium was insertionally inactivated at ssr3341, the mutants were not transformable, and were rendered non-phototactic compared to the wild type. The loss of motility was complemented by re-introduction of the wild-type gene, correlated with the re-establishment of type IV pili on the cell surface. Microarray analyses revealed a small set of genes with drastically reduced transcript levels in the knock-out mutant over wild-type cells. Among the most strongly affected genes are with slr1667, slr1668, slr2015, slr2016 and slr2018 five genes which belong to two operons that previously were shown to be involved in motility and controlled by the SYCRP1-cAMP receptor protein. This fact constitutes a link between cAMP signalling, motility, and the involvement of RNA-based regulation. This is the first report demonstrating a functional role of Hfq in cyanobacteria.
Keywords: comparative expression profiling
Overall design Microarray experiments were carried out as two-color hybridizations on custom designed 8-pack 15K Synechocystis sp. microarrays (AMADID 016989) from Agilent Technologies (Santa Clara, USA).
Contributor(s) Dienst D, Wallner T, Hess WR, Wilde A, Mollenkopf H
Citation(s) 18832319
Submission date Mar 04, 2008
Last update date Jul 14, 2014
Contact name Hans-Joachim Mollenkopf
Phone +49 30 28460 482
Organization name Max-Planck-Institute for Infection Biology
Lab Microarray/Genomics Core Facility
Street address Charit├ęplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platforms (1)
GPL6546 Agilent-016989 Synechocystis sp. PCC 6803 8x15K custom array
Samples (8)
GSM270625 wt 10/07 vs. hfq 10/07
GSM270632 hfq 10/07 vs. wt 10/07
GSM270633 wt 10/07 vs. hfq 04/07
BioProject PRJNA107587

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE10708_RAW.tar 35.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
Raw data provided as supplementary file
Raw data included within Sample table

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