Sequence variation within similar cis-elements promotes context-specific functions of two Drosophila GA-binding transcription factors (ChIP-seq data set)
Genome binding/occupancy profiling by high throughput sequencing
Summary
A key model for understanding how large transcription complexes are targeted is the Drosophila dosage compensation system in which the Male-Specific Lethal (MSL) transcription complex specifically identifies and regulates the male X-chromosome. MSL complex is targeted to GA-containing sequences, but the most well-studied GA-binding transcription factor, GAGA Associated Factor (GAF), does not physically associate with MSL complex. Instead the Chromatin Linked Adapter for MSL Proteins (CLAMP) zinc-finger protein specifically targets MSL complex to GA-rich sequences on the X-chromosome. Here, we compare the binding relationships of CLAMP, GAF, and the MSL3 dosage compensation complex protein using ChIP-seq.
Overall design
To map the occupancy of CLAMP and GAF relative to each other and to MSL complex at high resolution, we performed three to four replicates of ChIP-seq on all three factors in male Drosophila S2 cells under RNAi knockdown conditions of GFP (control), GAF (trl), and CLAMP.
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