GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE106759 Query DataSets for GSE106759
Status Public on Nov 10, 2017
Title Context-dependent effects of Acf1 assessed in high throughput
Organism Drosophila melanogaster
Experiment type Other
Summary We applied a multiplexed genomic tethering assay (Brueckner, Epigenetics Chromatin, 2016; PMID:27777628) to elucidate the function of ACF1, the large subunit of the ACF nucleosome sliding complex in drosophila Kc167 cells. Targeting a Gal4DBD-ACF1 fusion to several hundred integrated reporters revealed a context-dependent repressive effect which is modulated by gene activity and chromatin state.
Overall design TRIP assay in drosophila Kc167 cells combined with targeted recruitment of Gal4-Acf1 to integrated 5xGa4UAS-pMT-GFP reporters. Two technical replicate experiments of TRIP pool transfected with Gal4-Acf1, Gal4, Acf1 or Gal4-HP1 performed by day 2 after transient transfection.
Contributor(s) Brueckner L, Scacchetti A, Becker P, van Steensel B
Citation(s) 30456345
Submission date Nov 09, 2017
Last update date May 15, 2019
Contact name Bas van Steensel
Phone + 31 20 512 2040
Fax +31 20 669 1383
Organization name Netherlands Cancer Institute
Department division of Molecular Biology
Lab van Steensel group
Street address Plesmanlaan 121
City Amsterdam
ZIP/Postal code 1066 CX
Country Netherlands
Platforms (3)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (18)
GSM2849961 cDNA reads, Gal4-ACF1, repl 1
GSM2849962 cDNA reads, Gal4-ACF1, repl 2
GSM2849963 cDNA reads, Gal4, repl 1
BioProject PRJNA417865
SRA SRP124746

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106759_RAW.tar 10.0 Kb (http)(custom) TAR (of TXT)
GSE106759_TRIP_ACF1.csv.gz 82.9 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap