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Series GSE106719 Query DataSets for GSE106719
Status Public on Jun 28, 2018
Title Decreased TGFBR3/Betaglycan expression enhances the metastatic abilities of renal cell carcinoma cells through TGF-b-dependent and independent mechanisms
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary It is unclear how the loss of TGF-b signaling components affects metastatic abilities in clear cell renal cell carcinoma (ccRCC). In this study, we identified that low TGFBR3 expression in ccRCC cells enhanced primary tumor formation and lung metastasis. In the presence of TGFBR3, TGF-b2 decreased the aldehyde dehydrogenase (ALDH)-positive ccRCC cell population, in which renal cancer-initiating cells are enriched. Loss of TGFBR3 also enhanced cell migration in cell culture and induced expression of several mesenchymal markers in a TGF-b-independent manner. Increased lamellipodium formation by FAK-PI3K signaling was observed with TGFBR3 downregulation, and this contributed to TGF-b-independent cell migration in ccRCC cells. Taken together, our findings reveal that loss of TGFBR3 endows ccRCC cells with multiple metastatic abilities through TGF-b-dependent and independent pathways.
Overall design Target genes for TGF-b2 and TGFBR3 in ccRCC cells were identified.
Contributor(s) Nishida J, Miyazono K, Ehata S
Citation(s) 29391598
Submission date Nov 09, 2017
Last update date May 15, 2019
Contact name Shogo Ehata
Organization name The University of Tokyo
Department Graduate School of Medicine
Lab Deaprtment of Molecular Pathology
Street address Hongo 7-3-1, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-0033
Country Japan
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (4)
GSM2849318 RNA-seq_Caki-1-shNTC_no_treatment
GSM2849319 RNA-seq_Caki-1-shNTC_TGF-b2
GSM2849320 RNA-seq_Caki-1-shTGFBR3_B_no_treatment
BioProject PRJNA417759
SRA SRP124705

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Supplementary file Size Download File type/resource
GSE106719_shNTC_Tb2_vs_shTR3_B_Tb2.xlsx.gz 11.4 Mb (ftp)(http) XLSX
GSE106719_shNTC_mock_vs_shNTC_Tb2.xlsx.gz 11.3 Mb (ftp)(http) XLSX
GSE106719_shNTC_mock_vs_shTR3_B_mock.xlsx.gz 11.4 Mb (ftp)(http) XLSX
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