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| Status |
Public on Aug 17, 2018 |
| Title |
m6A-facilitated hippocampal learning and memory through Ythdf1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
N6-methyladenosine (m6A) is the most prevalent internal RNA modification in mammalian messenger RNAs (mRNAs). While m6A has been shown to mark groups of mRNAs for coordinated degradation in various physiological processes, the relevance of m6A in affecting translation remains to be determined in intact biological systems in vivo. Here we show that, through its reader protein Ythdf1, m6A promotes a pulse of protein synthesis of target transcripts in response to neuronal stimuli in the adult mouse hippocampus, thereby facilitating learning and memory processes. Mice with genetic deletion of the Ythdf1 gene (Ythdf1-/-) exhibit learning and memory defects, as well as impaired hippocampal synaptic transmission and long-term potentiation (LTP). Selective re-expression of Ythdf1 in the hippocampus of adult Ythdf1-/- mice fully rescues the behavioral and synaptic defects, while hippocampus-specific knockdown of Ythdf1 or Mettl3, the catalytic component of m6A methyltransferase complex, recapitulates the hippocampal deficiency in adult mice. At the molecular level, transcriptome-wide mapping of m6A sites and RNA binding sites of Ythdf1 on hippocampal mRNAs using crosslinking immunoprecipitation followed by high-throughput sequencing (CLIP-seq) uncovered key neuronal genes, including those involved in synaptic transmission and long-term potentiation. Nascent protein labelling and tether reporter assays in cultured hippocampal neurons revealed that Ythdf1 is critical for initiating a pulse of protein synthesis of target transcripts in a neuronal-stimulus-dependent manner. Collectively, our results uncover a pathway of mRNA m6A methylation in learning and memory, which is mediated through Ythdf1 in response to stimuli.
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| Overall design |
(1) The RNA binding targets of Ythdf1 were determined by anti-Ythdf1 antibody CLIP-seq in the adult mouse hippocampal tissue. (2) m6A-modified transcripts were mapped by anti-m6A antibody CLIP-seq using polyA RNA purified from the adult mouse hippocampus. (3) mRNA abundance in wild-type and Ythdf1-KO mouse hippocampus was determined by mRNA-seq. (4) Abundance of m6A-modified transcripts in mouse dentate gyrus before and after electroconvulsive treatment was determined by full length m6A-RIP-seq.
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| Contributor(s) |
Shi H, Lu Z, He C, Weng Y, Ming G, Song H, Zhou T |
| Citation(s) |
30401835 |
| |
| Submission date |
Nov 07, 2017 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Chuan He |
| E-mail(s) |
chuanhe@uchicago.edu
|
| Phone |
773-702-5061
|
| Organization name |
The University of Chicago
|
| Department |
Department of Chemistry
|
| Street address |
929 E 57th St., GCIS E319
|
| City |
Chicago |
| State/province |
ILLINOIS |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21626 |
NextSeq 550 (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA417392 |
| SRA |
SRP124463 |
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