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| Status |
Public on Mar 01, 2018 |
| Title |
Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and ibm1-3 trancriptomes in normal light and high light condition |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
n Arabidopsis and other species, anthocyanin accumulation specifically depends on light signaling. The CONSTITUTIVELY PHOTOMORPHOGENIC1/SUPPRESSOR OF PHYA-105 (COP1/SPA) complex is required for the control of anthocyanin biosynthesis in response to light, but the precise mechanism underlying this process is largely unknown. Here we report that Increase in BONSAI Methylation 1 (IBM1), a JmjC domain-containing histone demethylase, participates in the regulation of light-induced anthocyanin biosynthesis in Arabidopsis. Loss-of-function mutations of IBM1 lead to accelerated anthocyanin accumulation in high light. We further identified that IBM1 is directly associated with SPA1/2/3 chromatin in vivo, whereas knockout of IBM1 resulted in an elevated level of H3K9 methylation and DNA non-CG sites within SPA genes, thereby repressing their expression. Transgenic ibm1 plants overexpressing SPA1demonstrated that SPA1 was capable of complementing the mutant phenotype. Our results reveal a novel mechanism that controls the epigenetic regulation of anthocyanin biosynthesis and offer insight into how plant adapt to high light stress. This study could contribute to a better understanding of the linkage between light signaling and anthocyanin biosynthesis in plants
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| Overall design |
Total RNA was extracted using RNeasy Plant Mini Kit (QIAGEN) from Arabidopsis rosette leaves of 40-d-old ibm1-3 and WT plants grown under low light, or of 30-d-old plants treated by high light for 48 hours. mRNA samples were purified and subjected to Illumina sequencing as described in the Illumina mRNA sequencing sample preparation guide.
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| Contributor(s) |
Fan D, Luo K |
| Citation missing |
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| BioProject |
PRJNA415344 |
| Submission date |
Nov 06, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Di Fan |
| E-mail(s) |
cqrcwang@163.com
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| Organization name |
Southwest University
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| Street address |
NO.2 Tiansheng Road
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| City |
ChongQing |
| ZIP/Postal code |
400715 |
| Country |
China |
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| Platforms (2) |
| GPL21179 |
Illumina HiSeq 3000 (Arabidopsis thaliana) |
| GPL24025 |
BGISEQ-500 (Arabidopsis thaliana) |
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| Samples (6)
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| Relations |
| SRA |
SRP121401 |
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