GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE106522 Query DataSets for GSE106522
Status Public on Nov 13, 2017
Title HUH VP30 cells with and without interferon α and β treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain HUH VP30 samples for mRNA analysis with and with out interferon α and β treatment. Huh VP30 cells have been stably transfected with the Ebola VP30 gene, enabling replication of VP30-deficient Ebola virus. PubMed ID 18212124 and 19211761.
Overall design Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 250 U/ml of human interferon α and 250 U/ml of human interferon β(PBL Biosciences) with a multiplicity of 5 PFU per cell. Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points of 6, 12, and 18 hours. MERSCOV003.0P references the write up of the experimental procedure.
Contributor(s) Baric R, Sims A, Heller N, Waters KM, Eisfeld AJ, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 03, 2017
Last update date Jan 24, 2018
Contact name Natalie Heller
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (24)
GSM2839759 IFNa_a_HUHH0011_IFN_6h_1_RNA
GSM2839760 IFNa_a_HUHH0011_IFN_6h_2_RNA
GSM2839761 IFNa_a_HUHH0011_IFN_6h_3_RNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
BioProject PRJNA417084

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106522_RAW.tar 51.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap