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| Status |
Public on Nov 04, 2017 |
| Title |
Optogenetic neural stem cells microarray |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We elucidate the transcriptome effects of optogenetics on developing neural stem cells population.
The application of optogenetics in neural stem cell (NSC), allow photo-modulation of NSC in an activity-dependent manner. This provided spatio-temporal tool for studying activity dependent neurogenesis, including the potential of regulating the differentiation and maturation process of transplanted NSC. Currently, this is mainly driven by virally transfected of Channelrhodopsin-2 (ChR2) gene, which also requires high irradiance and complex in vitro stimulation systems. Additionally, despite the extensive application of optogenetics in neuroscience, the question of what transcriptome changes does optogenetic stimulation induced in developing NSCs have not been elucidated yet. To address these questions, we established a NSC line, expressing high light-sensitivity step-function opsin (SFO) variant of the chimeric channelrhodopsin, ChRFR(C167A) (~40x higher than ChR2), via the non-viral piggyBac transposon system. We set up a simple low-irradiance optogenetics stimulation-incubation system, which sufficiently induced depolarization in differentiating NSCs. We significantly enhance neurogenesis with low power optogenetic stimulation by 3 folds. Through microarray analysis, we have identified the genes and signaling pathways in axonal remodeling, synaptic plasticity and microenvironment modulation from optogenetic stimulation. Our results elucidate the transcriptome effects of optogenetics and the ability to drive neurogenesis with low irradiance optogenetics.
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| Overall design |
Two groups were compared between each other, control (non-stimulated) and stimulated group. Each group contains three replicates.
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| Contributor(s) |
Teh DB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 03, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Daniel Boon Loong Teh |
| E-mail(s) |
danielteh@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Street address |
28 Medical Drive, #05-COR
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| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (6)
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| GSM2839692 |
Differentiated Day 12_stimulate 30 minutes_1 |
| GSM2839693 |
Differentiated Day 12_stimulate 30 minutes_2 |
| GSM2839694 |
Differentiated Day 12_stimulate 30 minutes_3 |
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| Relations |
| BioProject |
PRJNA417074 |
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