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Series GSE10639 Query DataSets for GSE10639
Status Public on Feb 28, 2008
Title Differential expression of genes in pancreatic islets from high fat fed AKR/J and C57Bl/6J
Organism Mus musculus
Experiment type Expression profiling by array
Summary Increased fat intake is associated with obesity and insulin resistance. In some individuals, a failure of pancreatic b-cells to increase insulin production in response to the high demands of obesity leads to diabetes. We sought to determine whether the impaired b- cell adaptation in obesity is associated with differential expression of genes involved in b-cell expansion and intermediary metabolism. Two strains of inbred mice prone to obesity, C57Bl/6J and AKR/J, were fed regular rodent chow or high-fat diet, after which islet morphology, secretory function and gene expression were assessed. AKR/J had lower blood glucose and higher insulin levels compared with C57Bl/6J mice on regular rodent chow or high fat diet. Insulin secretion was 3.2 fold higher in AKR/J than C57Bl/6J mice following intraperitoneal glucose injection. Likewise, glucose-stimulated insulin secretion from isolated islets was higher in AKR/J. Additionally, islet mass was 1.4 fold greater in AKR/J compared with C57Bl/6J. To elucidate the factors associated with the differences in insulin, we analyzed the gene expression profiles in pancreatic islets in AKR/J and C57Bl/6J mice. Of 14,000 genes examined, 220 were up-regulated and 286 were down-regulated in islets from diet-induced obese AKR/J mice compared with C57Bl/6J mice. Key genes involved in islet signaling and metabolism, e.g. glucagon like peptide-1 receptor, sterol Co-A desaturase 1 & 2 and fatty acid desaturase 2 were upregulated in obese AKR/J mice. The expression of multiple extracellular matrix proteins was also increased in AKR/J mice, suggesting a role in modulation of islet mass. Functional analyses of differentially regulated genes hold promise for elucidating factors linking obesity to alterations in islet function.
Keywords: response to high fat diet
Overall design Microarray analyses were performed on quadruplicate RNA samples of pancreatic islets from AKR and Bl6 mice placed on high-fat diet for 3 months. Pancreases from two mice were combined to yield one sample of islet RNA. All protocols were conducted as described in the Affymetrix GeneChips Expression Analysis Technical Manual (Affymetrix, Santa Clara, CA) using 5 μg total RNA and GeneChip Mouse Expression Arrays MOE 430 (Affymetrix).
Contributor(s) Imai Y, Ahima R
Citation(s) 18854371
Submission date Feb 26, 2008
Last update date Feb 11, 2019
Contact name yumi imai
Phone 757-446-5708
Fax 757-446-7339
Organization name Eastern Virginia Medical School
Street address 799W Olney Road, LH 2156
City Norfolk
State/province VA
ZIP/Postal code 23507
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (8)
GSM268036 AKR/J No1
GSM268037 AKR/J No2
GSM268038 AKR/J No3
BioProject PRJNA107711

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Supplementary file Size Download File type/resource
GSE10639_RAW.tar 5.6 Mb (http)(custom) TAR (of CHP, TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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