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| Status |
Public on Nov 01, 2017 |
| Title |
An Elongin-Cullin-SOCS-box Complex Regulates Stress-induced Serotonergic Neuromodulation Stress-induced Serotonergic Neuromodulation |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Other
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| Summary |
Neuromodulatory cells transduce environmental information into long lasting behavioral responses. However, the mechanisms governing how defined neuronal cell types influence behavioral plasticity are difficult to characterize. Here we adapted the Translating Ribosome Affinity Purification (TRAP) approach in C. elegans to profile ribosome-associated mRNAs from three major tissues and the neuromodulatory dopaminergic and serotonergic cells. We identified elc-2, an Elongin C ortholog, specifically expressed in stress-sensing ADF serotonergic sensory neurons, and found that it plays a role in mediating long-lasting change in serotonin-dependent feeding behavior induced by heat stress. We demonstrate that ELC-2 and the von Hippel-Lindau protein VHL-1, components of an Elongin-Cullin-SOCS-box (ECS) E3 ubiquitin ligase, modulate this behavior after experiencing stress. Also, heat stress induces a transient redistribution of ELC-2, becoming more nuclearly enriched. Together, our results demonstrate dynamic regulation of an E3 ligase, and a role for an ECS complex in neuromodulation and control of lasting behavioral states.
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| Overall design |
RNA-seq data of three tissues and two neuronal subtypes using the Translating Ribosome Affinity Purification Technique in C elegans
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| Contributor(s) |
Gracida X, Calarco JA |
| Citation(s) |
29241538, 33127752 |
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| Submission date |
Oct 31, 2017 |
| Last update date |
Dec 15, 2020 |
| Contact name |
John Calarco |
| E-mail(s) |
john.calarco@utoronto.ca
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| Organization name |
University of Toronto
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| Department |
Cell and Systems Biology
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| Street address |
25 Harbord Street
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 3G5 |
| Country |
Canada |
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| Platforms (1) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA416495 |
| SRA |
SRP123154 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106374_Gracida_et_al_2017_TableS1.xlsx |
9.9 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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