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Series GSE106323 Query DataSets for GSE106323
Status Public on Oct 30, 2018
Title Hippocampal methylome of rats exposed to lead at different developmental stages
Organism Rattus norvegicus
Experiment type Methylation profiling by genome tiling array
Summary Developmental lead (Pb) exposure results in persistent cognitive/behavioral impairments as well as an elevated risk for developing a variety of diseases in later life. Environmental exposures during development can result in a variety of epigenetic changes, including alterations in DNA methylation, that can influence gene expression patterns and affect the function and development of the nervous system. The present promoter-based methylation microarray profiling study explored the extent to which developmental Pb exposure may modify the methylome of a brain region, hippocampus, known to be sensitive to the effects of Pb exposure. Male and female Long Evans rats were exposed to 0 ppm, 150 ppm, 375 ppm, or 750 ppm Pb through perinatal exposures (gestation through lactation), early postnatal exposures (birth through weaning), or long-term postnatal exposures (birth through postnatal day 55). Results showed a significant contribution of sex to the hippocampal methylome and effects of Pb exposure level, with non-linear dose response effects on methylation. Surprisingly, the developmental period of exposure contributed only a small amount of variance to the overall data and gene ontology (GO) analysis revealed the largest number of overrepresented GO terms in the groups with the lowest level of exposure. The highest number of significant differentially methylated regions was found in females exposed to Pb at the lowest exposure level. Our data reinforce the significant effect that low level Pb exposure may have on gene-specific DNA methylation patterns in brain and that this occurs in a sex-dependent manner.

NimbleGen Rat CpG Island plus RefSeq Promoter 720k array
 
Overall design Male and female Long-Evans rats were used for different Pb exposure paradigms: perinatal (PERI), early postnatal (EPN), and long-term postnatal (LPN) exposures. Controls (no lead exposure) were also included and all the comparisons were made to control group.
 
Contributor(s) Schneider JS
Citation(s) 29571894
Submission date Oct 30, 2017
Last update date Jan 29, 2019
Contact name Jay S Schneider
E-mail(s) jay.schneider@Jefferson.edu
Organization name Thomas Jefferson University
Department Anatomy,Pathology and Cell biology
Lab Room511
Street address 1020 Locust St
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platforms (1)
GPL16994 NimbleGen Rat CpG Island Plus RefSeq Promoter 720k array [100718_RN34_CpG_Refseq_Prom_MeDIP]
Samples (126)
GSM2835951 453912_A01
GSM2835952 453899_A02
GSM2835953 453956_A01
Relations
BioProject PRJNA416305

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106323_RAW.tar 3.1 Gb (http)(custom) TAR (of PAIR)
GSE106323_normalized_RANDOM_probes.txt.gz 1.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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