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| Status |
Public on Oct 30, 2018 |
| Title |
Hippocampal methylome of rats exposed to lead at different developmental stages |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Developmental lead (Pb) exposure results in persistent cognitive/behavioral impairments as well as an elevated risk for developing a variety of diseases in later life. Environmental exposures during development can result in a variety of epigenetic changes, including alterations in DNA methylation, that can influence gene expression patterns and affect the function and development of the nervous system. The present promoter-based methylation microarray profiling study explored the extent to which developmental Pb exposure may modify the methylome of a brain region, hippocampus, known to be sensitive to the effects of Pb exposure. Male and female Long Evans rats were exposed to 0 ppm, 150 ppm, 375 ppm, or 750 ppm Pb through perinatal exposures (gestation through lactation), early postnatal exposures (birth through weaning), or long-term postnatal exposures (birth through postnatal day 55). Results showed a significant contribution of sex to the hippocampal methylome and effects of Pb exposure level, with non-linear dose response effects on methylation. Surprisingly, the developmental period of exposure contributed only a small amount of variance to the overall data and gene ontology (GO) analysis revealed the largest number of overrepresented GO terms in the groups with the lowest level of exposure. The highest number of significant differentially methylated regions was found in females exposed to Pb at the lowest exposure level. Our data reinforce the significant effect that low level Pb exposure may have on gene-specific DNA methylation patterns in brain and that this occurs in a sex-dependent manner.
NimbleGen Rat CpG Island plus RefSeq Promoter 720k array
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| Overall design |
Male and female Long-Evans rats were used for different Pb exposure paradigms: perinatal (PERI), early postnatal (EPN), and long-term postnatal (LPN) exposures. Controls (no lead exposure) were also included and all the comparisons were made to control group.
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| Contributor(s) |
Schneider JS |
| Citation(s) |
29571894 |
| |
| Submission date |
Oct 30, 2017 |
| Last update date |
Jan 29, 2019 |
| Contact name |
Jay S Schneider |
| E-mail(s) |
jay.schneider@Jefferson.edu
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| Organization name |
Thomas Jefferson University
|
| Department |
Anatomy,Pathology and Cell biology
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| Lab |
Room511
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| Street address |
1020 Locust St
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16994 |
NimbleGen Rat CpG Island Plus RefSeq Promoter 720k array [100718_RN34_CpG_Refseq_Prom_MeDIP] |
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| Samples (126)
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| Relations |
| BioProject |
PRJNA416305 |
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